Antibiofilm Activity and Chemical Contents of Propolis Samples From Manisa-Turkey

Abstract

In this study, the inhibition of biofilm formation and the reduction of preformed or established biofilm by ethanol extract of propolis samples (EEP) obtained from Manisa-Turkey was investigated and their chemical composition was screened. The antibiofilm effect of the propolis extracts against biofilm forming bacteria (Listeria monocytogenes ATCC 7644, Methicillin Sensitive Staphylococcus aureus (clinical isolate) MSSA M20, S. aureus ATCC 33862, S. aureus ATCC 29213, Enterococcus faecalis ATCC 19433, Pseudomonas fluorescens ATCC 55241, Micrococcus luteus NRRL-B1013) was tested on 96-well polystyrene plates using crystal violet assay. Also, the antibacterial activity of EEP was evaluated according to Agar Well Diffusion method. Chemical composition of extracts was detected by Gas Chromatography-Mass Spectrometry (GC/MSD) analyze. The EEP samples exhibited good antibiofilm activity against bacteria. The maximum biofilm inhibition activity percentage of MP-1 (Manisa-Köprübaşı), MP-2 (Manisa-Demirci) and MP-3 (Manisa-Kula) were found as 89.4%, 80.0% and 89.0% for L. monocytogenes ATCC 7644 and 66.0%, 67.0% and 74.0% for MSSA M20, respectively. According to GC/ MSD analyze, triacontyl acetate was the major compound found in propolis extracts.