Synthesis of new 6-[4-(2-fluorophenylpiperazine-1-YL)]-3(2H)-pyridazinone-2-acethyl-2- (substitutedbenzal)hydrazone derivatives and evulation of their cytotoxic effects in liver and colon cancer cell lines

Abstract

In this study, seven new 3(2H)-pyridazinone derivatives expected to show cytotoxic activity in liver and colon cancer cell lines were synthesized. Their structures were confirmed by the IR, 1 H-NMR, 13 C-NMR spectra and elementary analyses. Compunds V 1 -V 7 were tested on HEP3B (liver cancer) and HTC116 (colon cancer) cell lines for cytotoxicity by using MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2H-tetrazolium] proliferation assay. Human fibroblast cells were used as safety control in these tests. 6-[4-(2-Fluorophenyl)piperazine-1-yl]-3(2H)-pyridazinone-2-acetyl-2-(2-chlorobenzal)hydrazone (compound V 3 ) was the most active agent with respect to HEP3B and HTC116 cell lines. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.