PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11499/45982
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Article Upadacitinib in Psoriatic Arthritis with Prior TNF-Inhibitor Failure: A 56-Week Real-World Study(Clinical & Exper Rheumatology, 2026) Bakay, U.; Duran, T. Izci; Bakay, O.S. KarstarliObjective Evidence on the long-term real-world effectiveness and safety of upadacitinib in TNFi-refractory psoriatic arthritis (PsA) remains limited. Our aim was to evaluate the real-world effectiveness and safety of upadacitinib in PsA patients with prior TNF-inhibitor failure. Methods This retrospective study included 47 patients with PsA (mean age 46.7 +/- 10.1 years, 59.6% female, mean disease duration 9.8 +/- 7.4 years). Patients received upadacitinib 15 mg daily and were followed up to 56 weeks. Clinical activity (DAPSA, BASDAI, ASDAS-CRP), ACR responses, PASI, patient-reported outcomes, and adverse events were assessed at baseline, week 12, 24, and 56. Intention-to-treat (ITT) analysis was the primary approach. Results Axial involvement was present in 74.5% and enthesitis in 85.1%. At week 12, ITT ACR20/50/70 response rates were 63.8%, 55.3%, and 31.9%, respectively. By week 56, ITT responses were 61.7%, 59.6%, and 51.1%. Significant improvements were also observed in DAPSA, BASDAI, ASDAS-CRP, and PASI scores (all p<0.001), with resolution of enthesitis in most patients. Adverse events occurred in 23.4% of patients, with 10.6% leading to discontinuation. Safety outcomes were consistent with the established profile of upadacitinib, with no new safety signals identified. Conclusion Upadacitinib demonstrated sustained efficacy across musculoskeletal and skin domains in PsA patients with prior inadequate response to TNF inhibitors, with a safety profile consistent with previous reports. These findings support its role as an effective treatment option in this difficult-to-treat population.Article TOE1 Is a β-Catenin Interacting Protein Regulating the Proliferation of Hematopoietic Cells through PAK2 Modulation(Cell Press, 2026-05-01) Palmer, David A.; Morgan, Rhys G.; Kim, Bomee; Sevim, Okan; Wagstaff, Megan; Goff, Aaron; Park, HyunArticle Therapeutic Effects of Stromal Stem Cells Derived from Ovarian Tissue in a Cyclophosphamide-Induced Rat Model of Ovarian Failure(Springer Heidelberg, 2026-04-29) Akgun, Mehpare; Unal, Murat Serkant; Onder, Elif; Tan, Semih; Altinbasak, FarukChemotherapeutic agents used in cancer treatment cause damage to both oocytes and granulosa cells, resulting in follicle loss and consequently premature ovarian failure. In this study the animals were randomly assigned to three groups: control (n = 6), chemotherapy (n = 6), and stem cell treatment (n = 6). Ovarian failure was induced in the chemotherapy and stem cell groups by intraperitoneal administration of cyclophosphamide (200 mg/kg) on days 1 and 8. Ovarian stromal stem cells (OSSCs) were isolated from the ovaries of 4-week-old donor rats (n = 2) using the explant culture method. On day 9, isolated stromal cells were transplanted bilaterally into the ovaries of rats in the stem cell group. Surface markers were analyzed by flow cytometry in OSSCs, and their adipogenic, osteogenic, and chondrogenic differentiation potentials were evaluated under appropriate in vitro conditions. The number of follicles and the morphological features of the ovarian tissue were histologically examined using hematoxylin and eosin (H&E) staining. Caspase 3 expression in ovarian tissue was analyzed using TUNEL and immunohistochemistry methods. Flow cytometry analysis of isolated ovarian stromal cells showed that CD54, CD90 and CD45 surface markers were expressed, while CD29 was expressed at a lower level. These findings confirmed the ability of OSSCs to differentiate into adipogenic, osteogenic, and chondrogenic lineages in vitro. Follicle counting, TUNEL and immunohistochemical analysis showed that treatment with ovarian stromal stem cells significantly reduced the number of atretic follicles and increased the number of normally developing follicles in the ovaries of chemotherapy-treated rats. Our research demonstrates the therapeutic effects of (allogeneic) ovarian stromal stem cells present in their niche on ovarian toxicity induced by chemotherapy, suggesting an alternative treatment option for approaches aimed at preserving fertility.Article The European Distal Renal Tubular Acidosis Registry: A 5-Year Analysis(Oxford Univ Press, 2026-03-06) Ozdemir, Gulsah; Veligratli, Faidra; Ruggiero, Barbara; Topaloglu, Rezan; Emma, Francesco; Giaccari, Marta; Hayes, WesleyBackground. Distal renal tubular acidosis (dRTA) is characterized by metabolic acidosis, growth failure, nephrocalcinosis, nephrolithiasis and chronic kidney disease (CKD). Previous retrospective data suggested improved outcome with adequate metabolic control, but confirmation from prospective data is lacking. Since 2019, the European dRTA registry has collected prospective data on patients with dRTA. Herein we present results of the first data analysis. Methods. The registry is hosted as a subregistry of the European Rare Kidney Disease Network (https://www.erknet.org/). In addition to the standard items of growth and plasma creatinine, additional data on plasma and urine biochemistries, genetics, treatment and clinical manifestations were collected from February 2019 through May 2024. Logistic regression analysis was used to identify predictors of CKD and impaired growth. Results. Of the 214 patients enrolled in the registry, only 22% were aged >18 years at the last visit. A genetic diagnosis was documented in 69% of patients. On average, low blood bicarbonate levels (<22 mmol/L) and hypercalciuria were observed in 42% and 25% of patients, respectively, independently of age. Multivariable analysis showed that height standard deviation score (SDS) was positively associated with serum bicarbonate levels (P = .008) and with early diagnosis (P = .005). Further modelling based on odds ratios indicated substantial odds of impaired growth for low serum bicarbonate levels. Patients aged >30 years had significantly lower estimated glomerular filtration rate (P < .001) and the overall prevalence of CKD stage >= 2 was 36%. Patients with SLC4A1 variants were at higher risk of CKD stage >1 (P = .013). Conclusion. Our results in this primarily paediatric cohort highlight the importance of metabolic control and support increasing the blood bicarbonate level for therapy to 24 mmol/L to improve growth. Compared with the overall population, patients with dRTA are at higher risk of CKD from childhood, particularly if they have underlying SLC4A1 variants.Article The Effects of Palbociclib and Ferulic Acid Combination on Cellular Processes in Breast Cancer(Springer, 2026-03-12) Ergezgin, Huriye; Bayav, Ibrahim; Tokgun, Pervin Elvan; Tomatir, Ayşe GayeBreast cancer remains a major cause of cancer-related morbidity and mortality worldwide, highlighting the urgent need for novel therapeutic approaches. In this study, we aimed to investigate the combined effects of Palbociclib (PLB), a cyclin-dependent kinase 4/6 (CDK4/6) inhibitor, and Ferulic Acid (FA), a natural antioxidant, on breast cancer cells (MCF-7 and MDA-MB231) as well as on non-tumorigenic breast epithelial cells (MCF-10 A). To this end, the effects of PLB and FA on cell viability, migration, cell cycle progression, oxidative stress, endoplasmic reticulum (ER) stress, mitochondrial membrane potential (MMP), and apoptosis were evaluated. MTT assay results demonstrated that both agents, alone and in combination, significantly suppressed proliferation in cancer cells, whereas their impact on MCF-10 A cells was comparatively limited. In addition, oxidative stress and lipid peroxidation levels increased markedly in cancer cells following the combination treatment, while this increase was relatively restricted in MCF-10 A cells. MMP was significantly reduced in cancer cell lines under combination treatment, but was largely preserved in MCF-10 A cells. Molecular analyses revealed upregulation of ER stress markers (PERK, ATF-6, CHOP, IRE1, GRP78) and pro-apoptotic genes (BAX, Caspases, CYC-C), along with downregulation of the anti-apoptotic gene BCL-2. Furthermore, phosphorylated retinoblastoma protein (pRB) levels decreased significantly, indicating enhanced cell cycle arrest and chemosensitivity. Taken together, these findings suggest that the combination of PLB and FA exerts a anti-cancer effect by simultaneously inducing oxidative and ER stress, disrupting mitochondrial integrity, and promoting apoptosis in breast cancer cells, while sparing non-tumorigenic cells. This selective cytotoxicity underscores the therapeutic potential of PLB and FA as a promising combinational strategy for breast cancer treatment.Article Temporal Bone Pneumatization and Tegmen Tympani Defects in Superior Semicircular Canal Dehiscence: Are They Related to Middle Cranial Fossa Depth(Via Medica, 2026) Cakmak, Vefa; Cakmak, PinarBackground: To investigate the relationship between superior semicircular canal dehiscence and tegmen defects, temporal bone pneumatization, and middle cranial fossa depth. Materials and methods: High-resolution temporal bone computed tomography scans obtained between January 2020 and August 2025 were retrospectively reviewed. The study included 88 patients with superior semicircular canal dehiscence and 88 control patients without this anatomical defect. On high-resolution computed tomography images, the presence ofsuperiorsemicircular canal dehiscence, tegmen tympani defect, tegmen mastoideum defect, middle cranial fossa depth, and temporal bone pneumatization were analyzed. Results: The frequency of tegmen tympani defect was 26.1% (n = 23) and that of tegmen mastoideum defect was 22.7% (n = 20) in patients with superior semicircular canal dehiscence. A statistically significant difference was found in the presence of tegmen tympani defect and tegmen mastoideum defect between patients with and without superior semicircular canal dehiscence. A significant increase in middle cranial fossa depth was observed in the presence of superior semicircular canal dehiscence and tegmen mastoideum defect. Correlation analysis revealed weak but meaningful positive relationships between superiorsemicircular canal dehiscence and tegmen tympani defect, tegmen mastoideum defect, and middle cranial fossa depth. Conclusions: Increased temporal bone pneumatization and greater middle cranial fossa depth may contribute to the development of superior semicircular canal dehiscence and associated tegmen defects. (Folia Morphol 2026; 85: e01726049)Article Surgical Treatment Outcomes of Elastofibroma Dorsi in 16 Patients with 22 Lesions(BMC, 2026-03-19) Bektaş, Mert; Aydemir, Ahmet Nadir; Kış, ArgünBackground Elastofibroma dorsi is a rare, slow-growing, benign soft tissue tumor typically located under the scapula. Although elastofibroma dorsi is a benign lesion, it may cause symptoms such as pain and restriction of movement, and its mass-like appearance can lead to considerable patient anxiety. We aimed to present patients diagnosed with elastofibroma dorsi who had clinical complaints and underwent surgical treatment, in conjunction with the literature in this study. Methods Our study retrospectively analyzed lesions surgically treated in the Orthopedics and Traumatology and Thoracic Surgery Clinics over the past five years, which were pathologically confirmed as elastofibroma dorsi. All patients were operated on in the prone position under general anesthesia, and all excised lesions underwent postoperative pathological examination. Results A total of 22 elastofibroma dorsi lesions were identified in 16 patients, including 6 patients with bilateral involvement. The mean age of the patients was 62.3 years (range, 46-76). Of these lesions, 13 (59.1%) were located on the right side and 9 (40.9%) on the left. The predominant presenting symptoms were pain, limitation of shoulder motion, cosmetic concerns, and the presence of a palpable mass. Lesion sizes ranged from 5 to 10 cm in diameter. Magnetic resonance imaging or computed tomography consistently demonstrated well-defined tumors situated posterolaterally in the chest wall, between the serratus anterior and latissimus dorsi muscles. All patients underwent marginal resection following anatomical dissection of the involved muscle groups. Postoperatively, seromas developed in 8 lesions (36.4%) and a hematoma in 1 lesion (4.5%). The seromas were successfully managed with aspiration and compression bandaging, while the hematoma required surgical reintervention. Conclusions Elastofibroma dorsi is a slowly growing benign lesion typically located beneath the inferior scapula. In symptomatic patients, surgical excision results in a marked improvement of symptoms. The most common surgical complication is the development of postoperative seroma.Article Stage-Dependent Expression of Autophagy-Related Genes in Patients With Knee Osteoarthritis(INT Inst Anticancer Research, 2026-04-28) Ok, Zeynep Dundar; Bozhkova, Martina; Petrov, Steliyan; Köseler, Aylin; Ök, Nusret; Gürbüz, Muhammed Erdi̇; Lengerova, Gergana; Dündar Ök, ZeynepBackground/Aim: Autophagy plays a crucial role in maintaining cellular homeostasis and has been implicated in the pathogenesis of knee osteoarthritis (OA). However, data on radiographic stage-dependent transcriptional variation of autophagy-related genes in patients with knee OA, particularly using peripheral blood samples, remain limited. The aim of this study was to evaluate whether disease severity was associated with stage-dependent changes in the expression of selected autophagy-related genes within a patient cohort. Patients and Methods: A total of 200 patients diagnosed with knee OA were included in the study. Disease severity was classified according to the Kellgren-Lawrence radiographic grading system. Peripheral blood samples were collected, and the expression levels of selected autophagy-related genes were analyzed using quantitative real-time polymerase chain reaction [autophagy-related 5 (ATG5), ATG7, unc-51-like kinase 1 (ULK1), microtubule-associated protein 1 light chain 3 beta (LC3B), WD repeat domain phosphoinositide-interacting protein 1 (WIPI1), neighbor of BRCA1 gene 1 (NBR1), forkhead box O3 (FOXO3), transcription factor EB (TFEB)]. Relative gene expression was calculated using the Delta Ct method, and comparisons were performed across radiographic stages. Associations between gene expression levels and systemic inflammatory markers were also assessed. Results: Significant stage-dependent differences were observed in the expression of ULK1, TFEB, WIPI1, and NBR1 (p<0.05), with higher Delta Ctvalues (reduced relative expression) in advanced radiographic stages compared with early-stage disease. In contrast, ATG5, ATG7, LC3B, and FOXO3 expression remained stable across radiographic stages. Furthermore, no significant associations were observed between expression of autophagy-related genes and systemic inflammatory status, as assessed by C-reactive protein levels. Conclusion: In patients with knee OA, regulatory and early autophagy-related genes exhibit radiographic stage-associated transcriptional alterations in peripheral blood, while expression of core autophagy machinery genes remain relatively stable. These findings suggest that disease severity is associated with selective transcriptional changes in autophagy-related pathways within the OA patient population and support further investigation of stage-dependent molecular patterns in knee OA.Article Simultaneous Detection of Primary Pulmonary Paraganglioma and a Mediastinal Mass: A Case Report(Ktcvs, 2026) Zenci, Eren; KıD, Argün; Kılıçarslan, EmelPrimary pulmonary paraganglioma (PPP) is an extremely rare neuroendocrine tumor. We present the case of a 71-year-old woman referred for dyspnea who was incidentally diagnosed with both a mediastinal mass and a pulmonary nodule. Chest computed tomography revealed an anterior mediastinal mass and a right-sided paracardiac pulmonary nodule. The mediastinal mass was excised via median sternotomy, and the pulmonary lesion was removed by wedge resection. Histopathological analysis identified the pulmonary lesion as a moderately differentiated paraganglioma, while the mediastinal mass consisted of ectopic thyroid tissue. Although the lesions were discovered simultaneously, their histopathological features were entirely unrelated, indicating a coincidental coexistence. Nevertheless, thorough histopathological examination is essential to exclude syndromic or metastatic associations. PPP should be considered in the differential diagnosis of pulmonary tumors, and surgical resection remains the mainstay of diagnosis and treatment.Article Rising Antimicrobial Resistance in Urinary Tract Infections in Türkiye: An Urgent Need for Update on Regional Treatment Strategies and for Enhanced Antimicrobial Stewardship Efforts(Springer, 2026-04-15) Akpınar, Anı; Aydemir, Şöhret; Aktaş, Elif; Can, Füsun; Vatansever, Cansel; Otlu, Barış; Öcal, DuyguPurpose Türkiye ranks among the highest in antibiotic consumption within OECD countries and faces growing challenges from multidrug-resistant uropathogens. Nationwide, resistance-guided treatment data remain scarce. The main objective of this work is to provide a comprehensive nationwide data on antibiotic resistance patterns among uropathogens to guide treat-ment options in urinary tract infections (UTIs). Methods A multicenter, retrospective observational study was conducted between 2021 and 2023 across 11 centers from 8 provinces representing all major geographical regions of Türkiye. Uropathogens isolated from adult patients with UTIs were analyzed to assess temporal trends and regional variability in antimicrobial resistance. Results In outpatients, resistance to commonly used oral agents (amoxicillin-clavulanate, ciprofloxacin) frequently exceeded 30%, peaking above 60% in Eastern and Southeastern regions. Nitrofurantoin and fosfomycin consistently demonstrated low resistance nationwide, supporting their continued use for uncomplicated UTIs. Aminoglycosides, including amikacin, main-tained low resistance rates across all regions, indicating potential for empirical use, though gradual year-on-year increases were observed. Multidrug and extensively drug-resistant isolates were most prevalent among elderly and inpatient popula-tions. In inpatients, Klebsiella pneumoniae showed particularly high resistance, with the last-line agent ceftazidime-avibac-tam resistance exceeding 60% in some regions. Conclusion For the treatment of uncomplicated UTIs, nitrofurantoin and fosfomycin remain reliable options, while amino-glycosides may be considered for complicated UTIs with caution. Pregnant women and elderly patients require special con-sideration due to limited alternatives. In inpatient settings, aminoglycosides remain viable empirical choices for suspected MDR infections. Strengthening antimicrobial stewardship and surveillance is critical to improving UTI management and combating antibiotic resistance in Türkiye.