Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/10506
Title: Genotoxic and cell-transforming effects of titanium dioxide nanoparticles
Authors: Demir, E.
Akça, Hakan
Turna, F.
Aksakal, S.
Burgucu, D.
Kaya, B.
Tokgün, O.
Keywords: Comet assay
HEK293 and NIH/3T3 cells
Micronucleus assay
Nano-TiO2
Soft-agar assay
nanomaterial
titanium dioxide nanoparticle
metal nanoparticle
mutagenic agent
titanium
titanium dioxide
bioassay
biotransformation
cell organelle
dose-response relationship
enzyme activity
genetic analysis
genotoxicity
oxide
particulate matter
3T3 cell line
animal cell
Article
cell transformation
comet assay
controlled study
DNA damage
environmental exposure
HEK293 cell line
human
human cell
micronucleus test
nanotechnology
nonhuman
transmission electron microscopy
toxicity
Comet Assay
HEK293 Cells
Humans
Metal Nanoparticles
Micronucleus Tests
Mutagens
Titanium
Publisher: Academic Press Inc.
Abstract: The in vitro genotoxic and the soft-agar anchorage independent cell transformation ability of titanium dioxide nanoparticles (nano-TiO2) and its microparticulated form has been evaluated in human embryonic kidney (HEK293) and in mouse embryonic fibroblast (NIH/3T3) cells. Nano-TiO2 of two different sizes (21 and 50nm) were used in this study. The comet assay, with and without the use of FPG enzyme, the micronucleus assay and the soft-agar colony assay were used. For both the comet assay and the frequency of micronuclei a statistically significant induction of DNA damage, was observed at the highest dose tested (1000µg/mL). No oxidative DNA damage induction was observed when the comet assay was complemented with the use of FPG enzyme. Furthermore, long-term exposure to nano-TiO2 has also proved to induce cell-transformation promoting cell-anchorage independent growth in soft-agar. Results were similar for the two nano-TiO2 sizes. Negative results were obtained when the microparticulated form of TiO2 was tested, indicating the existence of important differences between the microparticulated and nanoparticulated forms. As a conclusion it should be indicated that the observed genotoxic/tranforming effects were only detected at the higher dose tested (1000µg/mL) what play down the real risk of environmental exposures to this nanomaterial. © 2014 Elsevier Inc.
URI: https://hdl.handle.net/11499/10506
https://doi.org/10.1016/j.envres.2014.10.032
ISSN: 0013-9351
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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