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https://hdl.handle.net/11499/10506
Title: | Genotoxic and cell-transforming effects of titanium dioxide nanoparticles | Authors: | Demir, E. Akça, Hakan Turna, F. Aksakal, S. Burgucu, D. Kaya, B. Tokgün, O. |
Keywords: | Comet assay HEK293 and NIH/3T3 cells Micronucleus assay Nano-TiO2 Soft-agar assay nanomaterial titanium dioxide nanoparticle metal nanoparticle mutagenic agent titanium titanium dioxide bioassay biotransformation cell organelle dose-response relationship enzyme activity genetic analysis genotoxicity oxide particulate matter 3T3 cell line animal cell Article cell transformation comet assay controlled study DNA damage environmental exposure HEK293 cell line human human cell micronucleus test nanotechnology nonhuman transmission electron microscopy toxicity Comet Assay HEK293 Cells Humans Metal Nanoparticles Micronucleus Tests Mutagens Titanium |
Publisher: | Academic Press Inc. | Abstract: | The in vitro genotoxic and the soft-agar anchorage independent cell transformation ability of titanium dioxide nanoparticles (nano-TiO2) and its microparticulated form has been evaluated in human embryonic kidney (HEK293) and in mouse embryonic fibroblast (NIH/3T3) cells. Nano-TiO2 of two different sizes (21 and 50nm) were used in this study. The comet assay, with and without the use of FPG enzyme, the micronucleus assay and the soft-agar colony assay were used. For both the comet assay and the frequency of micronuclei a statistically significant induction of DNA damage, was observed at the highest dose tested (1000µg/mL). No oxidative DNA damage induction was observed when the comet assay was complemented with the use of FPG enzyme. Furthermore, long-term exposure to nano-TiO2 has also proved to induce cell-transformation promoting cell-anchorage independent growth in soft-agar. Results were similar for the two nano-TiO2 sizes. Negative results were obtained when the microparticulated form of TiO2 was tested, indicating the existence of important differences between the microparticulated and nanoparticulated forms. As a conclusion it should be indicated that the observed genotoxic/tranforming effects were only detected at the higher dose tested (1000µg/mL) what play down the real risk of environmental exposures to this nanomaterial. © 2014 Elsevier Inc. | URI: | https://hdl.handle.net/11499/10506 https://doi.org/10.1016/j.envres.2014.10.032 |
ISSN: | 0013-9351 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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