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https://hdl.handle.net/11499/10656
Title: | Ameliorative potential of Lavandula stoechas in metabolic syndrome via multitarget interactions | Authors: | Kulabas, S.S. Ipek, H. Tufekci, A.R. Arslan, Şevki Demirtas, I. Ekren, R. Sezerman, U. |
Keywords: | Gluconeogenesis Glucose uptake Insulin resistance Lavandula stoechas Lipolysis Transcriptome 4 hydroxybenzoic acid antiinflammatory agent apigenin caffeic acid cyclooxygenase 2 ferulic acid glucose 6 phosphatase inducible nitric oxide synthase insulin interleukin 1beta isoprenaline Lavandula stoechas extract lipoprotein lipase luteolin messenger RNA metformin nitric oxide plant extract rosmarinic acid salicylic acid unclassified drug vanillic acid glucose peroxisome proliferator activated receptor gamma phosphoenolpyruvate carboxylase kinase protein kinase B protein serine threonine kinase adipocyte cell line animal cell antiinflammatory activity Article C2C12 cell line controlled study drug mechanism gluconeogenesis glucose transport H4-II-E cell line high performance liquid chromatography in vitro study lavender lipolysis metabolic syndrome X myotube nonhuman RAW 264.7 cell line time of flight mass spectrometry transcriptomics aerial plant part animal cell line cell survival drug effect gene expression regulation genetics metabolism mouse rat tumor cell line Animals Cell Line Cell Line, Tumor Cell Survival Cyclooxygenase 2 Gene Expression Regulation Glucose Glucose-6-Phosphatase Interleukin-1beta Lavandula Metabolic Syndrome Mice Nitric Oxide Nitric Oxide Synthase Type II Plant Components, Aerial Plant Extracts PPAR gamma Protein-Serine-Threonine Kinases Proto-Oncogene Proteins c-akt Rats |
Publisher: | Elsevier Ireland Ltd | Abstract: | Ethnopharmacological importance: Decoction and infusion prepared from aerial parts of Lavandula stoechas L. (L. stoechas) have been traditionally used as remedy against several components of metabolic syndrome (MetS) and associated disorders including type II diabetes and cardiovascular diseases by Anatolian people. Aim of the study: The aim is to elucidate the potential ameliorative effects of L. stoechas aqueous extracts on insulin resistance and inflammation models through multitarget in vitro approaches and also to elucidate mechanism of action by analyzing transcriptional and metabolic responses. Materials and methods: An aqueous extract was prepared and fractionated to give rise to ethyl acetate (EE) and butanol (BE) extracts. The anti-insulin resistance effects of BE and EE were evaluated on palmitate induced insulin resistance model of H4IIE, C2C12 and 3T3L1 cells by using several metabolic parameters. Specifically, whole genome transcriptome analysis was performed by using microarray over 55.000 genes in control, insulin resistant and EE (25 µg/mL) treated insulin resistant H4IIE cells. Anti-inflammatory effects of both extracts were analyzed in LPS-stimulated RAW264.7 macrophages. Results: Both EE and BE at low doses (25–50 µg/mL) significantly decreased hepatic gluconeogenesis in H4IIE cell line by suppressing the expression of PEPCK and G6Pase. In C2C12 myotubes, both extracts increased the insulin stimulated glucose uptake more effectively than metformin. Both extracts decreased the isoproterenol induced lipolysis in 3T3L1 cell line. Moreover, they also effectively increased the expression of lipoprotein lipase protein level in insulin resistant myotubes at low doses. EE increased the protein level of PPAR? and stimulated the activation AKT in insulin resistant H4IIE and C2C12 cell lines. The results obtained from biochemical assays, mRNA/protein studies and whole genome transcriptome analyses were found to be complementary and provided support for the hypothesis that EE might be biologically active against insulin resistance and act through the inhibition of liver gluconeogenesis and AKT activation. Besides, LPS induced inflammation in RAW264.7 macrophages was mainly inhibited by EE through suppression of iNOS/NO signaling, IL1ß and COX-2 genes. HPLC-TOF/MS analysis of EE of L. stoechas mainly resulted in caffeic acid, apigenin, luteolin, rosmarinic acid and its methyl ester, 4-hydroxybenzoic acid, vanillic acid, ferrulic acid and salicylic acid. Conclusion: Data suggest that EE of L. stoechas contains phytochemicals that can be effective in the treatment/prevention of insulin resistance and inflammation. These results validate the traditional use of L. stoechas in Anatolia against several metabolic disorders including metabolic syndrome. © 2018 | URI: | https://hdl.handle.net/11499/10656 https://doi.org/10.1016/j.jep.2018.04.043 |
ISSN: | 0378-8741 |
Appears in Collections: | Fen-Edebiyat Fakültesi Koleksiyonu PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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