Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/10656
Title: Ameliorative potential of Lavandula stoechas in metabolic syndrome via multitarget interactions
Authors: Kulabas, S.S.
Ipek, H.
Tufekci, A.R.
Arslan, Şevki
Demirtas, I.
Ekren, R.
Sezerman, U.
Keywords: Gluconeogenesis
Glucose uptake
Insulin resistance
Lavandula stoechas
Lipolysis
Transcriptome
4 hydroxybenzoic acid
antiinflammatory agent
apigenin
caffeic acid
cyclooxygenase 2
ferulic acid
glucose 6 phosphatase
inducible nitric oxide synthase
insulin
interleukin 1beta
isoprenaline
Lavandula stoechas extract
lipoprotein lipase
luteolin
messenger RNA
metformin
nitric oxide
plant extract
rosmarinic acid
salicylic acid
unclassified drug
vanillic acid
glucose
peroxisome proliferator activated receptor gamma
phosphoenolpyruvate carboxylase kinase
protein kinase B
protein serine threonine kinase
adipocyte cell line
animal cell
antiinflammatory activity
Article
C2C12 cell line
controlled study
drug mechanism
gluconeogenesis
glucose transport
H4-II-E cell line
high performance liquid chromatography
in vitro study
lavender
lipolysis
metabolic syndrome X
myotube
nonhuman
RAW 264.7 cell line
time of flight mass spectrometry
transcriptomics
aerial plant part
animal
cell line
cell survival
drug effect
gene expression regulation
genetics
metabolism
mouse
rat
tumor cell line
Animals
Cell Line
Cell Line, Tumor
Cell Survival
Cyclooxygenase 2
Gene Expression Regulation
Glucose
Glucose-6-Phosphatase
Interleukin-1beta
Lavandula
Metabolic Syndrome
Mice
Nitric Oxide
Nitric Oxide Synthase Type II
Plant Components, Aerial
Plant Extracts
PPAR gamma
Protein-Serine-Threonine Kinases
Proto-Oncogene Proteins c-akt
Rats
Publisher: Elsevier Ireland Ltd
Abstract: Ethnopharmacological importance: Decoction and infusion prepared from aerial parts of Lavandula stoechas L. (L. stoechas) have been traditionally used as remedy against several components of metabolic syndrome (MetS) and associated disorders including type II diabetes and cardiovascular diseases by Anatolian people. Aim of the study: The aim is to elucidate the potential ameliorative effects of L. stoechas aqueous extracts on insulin resistance and inflammation models through multitarget in vitro approaches and also to elucidate mechanism of action by analyzing transcriptional and metabolic responses. Materials and methods: An aqueous extract was prepared and fractionated to give rise to ethyl acetate (EE) and butanol (BE) extracts. The anti-insulin resistance effects of BE and EE were evaluated on palmitate induced insulin resistance model of H4IIE, C2C12 and 3T3L1 cells by using several metabolic parameters. Specifically, whole genome transcriptome analysis was performed by using microarray over 55.000 genes in control, insulin resistant and EE (25 µg/mL) treated insulin resistant H4IIE cells. Anti-inflammatory effects of both extracts were analyzed in LPS-stimulated RAW264.7 macrophages. Results: Both EE and BE at low doses (25–50 µg/mL) significantly decreased hepatic gluconeogenesis in H4IIE cell line by suppressing the expression of PEPCK and G6Pase. In C2C12 myotubes, both extracts increased the insulin stimulated glucose uptake more effectively than metformin. Both extracts decreased the isoproterenol induced lipolysis in 3T3L1 cell line. Moreover, they also effectively increased the expression of lipoprotein lipase protein level in insulin resistant myotubes at low doses. EE increased the protein level of PPAR? and stimulated the activation AKT in insulin resistant H4IIE and C2C12 cell lines. The results obtained from biochemical assays, mRNA/protein studies and whole genome transcriptome analyses were found to be complementary and provided support for the hypothesis that EE might be biologically active against insulin resistance and act through the inhibition of liver gluconeogenesis and AKT activation. Besides, LPS induced inflammation in RAW264.7 macrophages was mainly inhibited by EE through suppression of iNOS/NO signaling, IL1ß and COX-2 genes. HPLC-TOF/MS analysis of EE of L. stoechas mainly resulted in caffeic acid, apigenin, luteolin, rosmarinic acid and its methyl ester, 4-hydroxybenzoic acid, vanillic acid, ferrulic acid and salicylic acid. Conclusion: Data suggest that EE of L. stoechas contains phytochemicals that can be effective in the treatment/prevention of insulin resistance and inflammation. These results validate the traditional use of L. stoechas in Anatolia against several metabolic disorders including metabolic syndrome. © 2018
URI: https://hdl.handle.net/11499/10656
https://doi.org/10.1016/j.jep.2018.04.043
ISSN: 0378-8741
Appears in Collections:Fen-Edebiyat Fakültesi Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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