Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/10787
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dc.contributor.authorŞirin, D.Y.-
dc.contributor.authorKaplan, N.-
dc.contributor.authorYılmaz, I.-
dc.contributor.authorKaraarslan, N.-
dc.contributor.authorÖzbek, H.-
dc.contributor.authorAkyuva, Y.-
dc.contributor.authorKaya, Y.E.-
dc.date.accessioned2019-08-16T13:32:57Z
dc.date.available2019-08-16T13:32:57Z
dc.date.issued2018-
dc.identifier.issn1792-0981-
dc.identifier.urihttps://hdl.handle.net/11499/10787-
dc.identifier.urihttps://doi.org/10.3892/etm.2018.5943-
dc.description.abstractThe aim of the present study was to investigate the effects of three different formulations of hyaluronic acid (HA): Low molecular weight (MW) Sinovial One®, medium MW Viscoplus® and high MW Durolane®, on chondrocyte proliferation and collagen type II (COL2A1), hypoxia-inducible factor 1? (HIF-1?) and chondroadherin (CHAD) expression in primary chondrocyte cultures. Standard primary chondrocyte cultures were established from osteochondral tissues surgically obtained from 6 patients with gonarthrosis. Cell morphology was evaluated using an inverted light microscope; cell proliferation was determined with a MTT assay and confirmed with acridine orange/propidium iodide staining. Levels of CHAD, COL2A1 and HIF-1? expression were assessed using specific TaqMan gene expression assays. The results demonstrated the positive effect of HA treatment on cell proliferation, which was independent from the MW. COL2A1 expression increased in the medium and high MW HA treated groups. It was observed that HIF-1? expression increased in the high MW treated group alone. CHAD expression increased only in the medium MW HA treated group. Evaluation of gene expression revealed that levels of expression increased as the duration of HA application increased, in the medium and high MW HA treated groups. In terms of increased viability and proliferation, a longer duration of HA application was more effective. Taken together, it may be concluded that the administration of medium and high MW HA may be a successful way of treating diseases affecting chondrocytes in a clinical setting. © 2018, Spandidos Publications. All rights reserved.en_US
dc.language.isoenen_US
dc.publisherSpandidos Publicationsen_US
dc.relation.ispartofExperimental and Therapeutic Medicineen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectChondroadherinen_US
dc.subjectChondrocytesen_US
dc.subjectCollagen type IIen_US
dc.subjectHyaluronic aciden_US
dc.subjectHypoxia-inducible factor 1?en_US
dc.subjectchondroadherinen_US
dc.subjectcollagen type 2en_US
dc.subjecthyaluronic aciden_US
dc.subjecthypoxia inducible factor 1alphaen_US
dc.subjectproteinen_US
dc.subjectsinovial oneen_US
dc.subjectunclassified drugen_US
dc.subjectviscoplusen_US
dc.subjectadulten_US
dc.subjectArticleen_US
dc.subjectcell proliferationen_US
dc.subjectcell structureen_US
dc.subjectcell viabilityen_US
dc.subjectchondrocyteen_US
dc.subjectchondrocyte cultuteen_US
dc.subjectclinical articleen_US
dc.subjectcontrolled studyen_US
dc.subjectfemaleen_US
dc.subjectfluorescence microscopyen_US
dc.subjectgene expressionen_US
dc.subjectgene expression assayen_US
dc.subjecthumanen_US
dc.subjecthuman tissueen_US
dc.subjectin vivo studyen_US
dc.subjectknee osteoarthritisen_US
dc.subjectmaleen_US
dc.subjectmicroscopyen_US
dc.subjectmiddle ageden_US
dc.subjectmolecular weighten_US
dc.subjectMTT assayen_US
dc.subjectprimary cultureen_US
dc.subjectreal time polymerase chain reactionen_US
dc.subjectreverse transcription polymerase chain reactionen_US
dc.subjectstainingen_US
dc.titleThe association between different molecular weights of hyaluronic acid and CHAD, HIF-1?, COL2A1 expression in chondrocyte culturesen_US
dc.typeArticleen_US
dc.identifier.volume15en_US
dc.identifier.issue5en_US
dc.identifier.startpage4205
dc.identifier.startpage4205en_US
dc.identifier.endpage4212en_US
dc.identifier.doi10.3892/etm.2018.5943-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.pmid29849772en_US
dc.identifier.scopus2-s2.0-85044444457en_US
dc.identifier.wosWOS:000431850300018en_US
dc.identifier.scopusqualityQ2-
dc.ownerPamukkale University-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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