HPLC analysis and antioxidant, antibacterial and cytotoxicity activities of various solvent extracts of Erysimum kotschyanum Gay. (Brassicaceae)

Abstract

Erysimum is the second richest genus in Brassicaceae. Erysimum species have been used in some phytochemical and biological activities studies, as well as in the taxonomic, floristic and genetic works. In this study, some biological activities and phenolic contents of various solvents extracts (ethanol, acetone and distileted water) of Erysimum kotschyanum Gay. were evaluated..The antioxidant activity of the extracts was evaluated by ß-carotene/linoleic acid, ferric reducing power assays (FRAP), radical scavenging assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2'-azino-bis-3-ethyl benzthiazoline-6-sulphonic acid (ABTS) and total phenolic and flavonoid contents in the extracts were determined. The brine shrimp lethality test was used to determine the cytotoxic activity of E. kotschyanum extracts and also, antibacterial potential of extracts obtained. Antioxidant activity was determined as ß-carotene/linoleic acid (%80.47±1.83), DPPH (IC50: 0.29±0.03) and ABTS (IC50: 47.15±0.74), ferric reducing antioxidant power (FRAP) (IC50: 18.79±0.15), total phenolic and total flavonoid contents was found as (4.883±0.47 mgGAE/g) and (93.322±1.57 mgQE/g), respectively, and then 9 different phenolic compounds in ethanol extracts was carried out by HPLC. Also, ethanol extract's antimicrobial activity (17±1 zone diameter) and cytotoxic activity (Brine Shrimp LC50: 315.48) of extracts was calculated. According the results, the extract of E. kotschyanum may be considered as a potential source of biological agents.. © 2018 Chemical Society of Pakistan. All rights reserved.