Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/10939
Title: Characterizing the Association Between Toll-like Receptor Subtypes and Nephrolithiasis With Renal Inflammation in an Animal Model
Authors: Olcucu, M.T.
Teke, K.
Yalcin, S.
Olcucuoglu, E.
Caner, Vildan
Türk, Nilay Şen
Tuncay, Ömer Levent
Keywords: calcium oxalate
ethylene glycol
messenger RNA
oxalic acid
toll like receptor
toll like receptor 1
toll like receptor 11
toll like receptor 2
toll like receptor 3
toll like receptor 6
toll like receptor 7
animal cell
animal experiment
animal model
animal tissue
Article
bilateral nephrectomy
bladder stone
controlled study
disease association
histopathology
hyperoxaluria
in vivo study
kidney biopsy
kidney injury
male
microscopy
nephritis
nephrolithiasis
nonhuman
priority journal
protein expression
rat
real time polymerase chain reaction
stone formation
urine level
urine sampling
animal
classification
disease model
physiology
Wistar rat
Animals
Disease Models, Animal
Kidney Calculi
Male
Nephritis
Rats, Wistar
Toll-Like Receptors
Publisher: Elsevier Inc.
Abstract: Objective To show experimentally induced renal stone disease and to evaluate secondary inflammatory responses in vivo, and to characterize changes in the expression of Toll-like receptor (TLR) subtypes in this model. Methods Twenty 5- to 6-week-old male Wistar rats were divided into control and hyperoxaluria groups (n = 10 per group) and were supplied with normal water or 1% ethylene glycol, respectively, for 16 weeks. The animals were then placed in metabolic cages, and urine was collected for a 24-hour urine oxalate level evaluation. Following sacrifice, rats were subjected to bilateral nephrectomy and both kidneys were histopathologically evaluated. A 1-mm3 biopsy section from the right kidney of each rat was subjected to real-time polymerase chain reaction of the TLR expression. Results At the end of week 16, the hyperoxaluria group had a higher mean 24-hour urine oxalate level (1.91) than the control group (0.29) (P <.05) and a remarkably increased deposition of renal CaOx crystals (15/20) than the control group (0/20) (P <.05), which was universally accompanied by inflammation (15/15). Twelve and no rats in the hyperoxaluria and control groups, respectively, had macroscopically visible renal pelvic stones (P <.05). Quantitative real-time polymerase chain reaction revealed significant decreases in the expression of several TLRs, particularly TLR11 and TLR7. Decreases in TLR1, TLR3, and TLR6 expressions and an increase in the TLR2 expression did not differ significantly between the groups. Conclusion We believe that is the first evaluation of TLR expression associated with renal stone formation in an animal model of inflammation. These results might lead to novel TLR-based treatments for nephrolithiasis and related inflammatory renal damage. © 2017 Elsevier Inc.
URI: https://hdl.handle.net/11499/10939
https://doi.org/10.1016/j.urology.2017.09.026
ISSN: 0090-4295
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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