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https://hdl.handle.net/11499/10939
Title: | Characterizing the Association Between Toll-like Receptor Subtypes and Nephrolithiasis With Renal Inflammation in an Animal Model | Authors: | Olcucu, M.T. Teke, K. Yalcin, S. Olcucuoglu, E. Caner, Vildan Türk, Nilay Şen Tuncay, Ömer Levent |
Keywords: | calcium oxalate ethylene glycol messenger RNA oxalic acid toll like receptor toll like receptor 1 toll like receptor 11 toll like receptor 2 toll like receptor 3 toll like receptor 6 toll like receptor 7 animal cell animal experiment animal model animal tissue Article bilateral nephrectomy bladder stone controlled study disease association histopathology hyperoxaluria in vivo study kidney biopsy kidney injury male microscopy nephritis nephrolithiasis nonhuman priority journal protein expression rat real time polymerase chain reaction stone formation urine level urine sampling animal classification disease model physiology Wistar rat Animals Disease Models, Animal Kidney Calculi Male Nephritis Rats, Wistar Toll-Like Receptors |
Publisher: | Elsevier Inc. | Abstract: | Objective To show experimentally induced renal stone disease and to evaluate secondary inflammatory responses in vivo, and to characterize changes in the expression of Toll-like receptor (TLR) subtypes in this model. Methods Twenty 5- to 6-week-old male Wistar rats were divided into control and hyperoxaluria groups (n = 10 per group) and were supplied with normal water or 1% ethylene glycol, respectively, for 16 weeks. The animals were then placed in metabolic cages, and urine was collected for a 24-hour urine oxalate level evaluation. Following sacrifice, rats were subjected to bilateral nephrectomy and both kidneys were histopathologically evaluated. A 1-mm3 biopsy section from the right kidney of each rat was subjected to real-time polymerase chain reaction of the TLR expression. Results At the end of week 16, the hyperoxaluria group had a higher mean 24-hour urine oxalate level (1.91) than the control group (0.29) (P <.05) and a remarkably increased deposition of renal CaOx crystals (15/20) than the control group (0/20) (P <.05), which was universally accompanied by inflammation (15/15). Twelve and no rats in the hyperoxaluria and control groups, respectively, had macroscopically visible renal pelvic stones (P <.05). Quantitative real-time polymerase chain reaction revealed significant decreases in the expression of several TLRs, particularly TLR11 and TLR7. Decreases in TLR1, TLR3, and TLR6 expressions and an increase in the TLR2 expression did not differ significantly between the groups. Conclusion We believe that is the first evaluation of TLR expression associated with renal stone formation in an animal model of inflammation. These results might lead to novel TLR-based treatments for nephrolithiasis and related inflammatory renal damage. © 2017 Elsevier Inc. | URI: | https://hdl.handle.net/11499/10939 https://doi.org/10.1016/j.urology.2017.09.026 |
ISSN: | 0090-4295 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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