Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/11020
Title: Eremurus spectabilis, rich source of isoorientin: Isolation, quantification and anti-cancer activity on SH-SY5Y neuroblastoma cells
Authors: Karaoğlan, E.S.
Gündoğdu, G.
Seçme, Mücahit
Şenol, O.
Miloğlu, F.D.
Dodurga, Y.
Tüfekçi, A.R.
Keywords: Eremurus spectabilis BIEB
HPLC
Isolation
Isoorientin
Neuroblastoma
XTT
Publisher: Bentham Science Publishers B.V.
Abstract: Background: Eremurus spectabilis BIEB. (Liliaceae) is an edible and medicinal plant in Turkey. Introduction: This study was designed to isolate and quantify isoorientin in leaves of E. spectabilis and exhibit its effect on SH-SY5Y neuroblastoma cell line. Methods: Purity and identification of isoorientin were evaluated by 1D-NMR, 2D-NMR and Q-TOF were isolated from E. spectabilis via column chromatography. An HPLC method was also developed and validated for isoorientin. Results: Quantitative measurements indicated that contents of isoorientin in E. spectabilis leaves wereY 81.01 mg/g and MeOH extract were 23.75 mg/gr. All measurements were performed at 350 nm. Anticancer activity was investigated on cell culture. IC50 doses of isoorientin were detected as 250 µM at the 48th hour in SH-SY5Y cells by XTT assay. Real-time PCR analysis in SH-SY5Y cells showed that CCND1, CDK6, casp-9, Bax, ATR, Bcl-2, CHEK1 and CHEK2, expressions significantly reduced in experimental group when compared with the control group. p53, p21, caspase-3, caspase-8, Bcl-2, ATM and ERCC1 expressions increased in the experimental group when compared with the control group (P <0.05). Conclusion: Measurements revealed that E. spectabilis contains high amount of isoorientin and can be used as a new source of this compound. In addition to this, isoorientin affects cell proliferation of neuroblastoma cells by cell cycle control and apoptosis gene expression and it could be used as a therapeutic agent, however, more studies must be performed to clarify its mechanism. © 2018 Bentham Science Publishers.
URI: https://hdl.handle.net/11499/11020
https://doi.org/10.2174/1573412913666170822162527
ISSN: 1573-4129
Appears in Collections:Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Show full item record



CORE Recommender

SCOPUSTM   
Citations

2
checked on Nov 16, 2024

WEB OF SCIENCETM
Citations

2
checked on Nov 21, 2024

Page view(s)

76
checked on Aug 24, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.