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https://hdl.handle.net/11499/11020
Title: | Eremurus spectabilis, rich source of isoorientin: Isolation, quantification and anti-cancer activity on SH-SY5Y neuroblastoma cells | Authors: | Karaoğlan, E.S. Gündoğdu, G. Seçme, Mücahit Şenol, O. Miloğlu, F.D. Dodurga, Y. Tüfekçi, A.R. |
Keywords: | Eremurus spectabilis BIEB HPLC Isolation Isoorientin Neuroblastoma XTT |
Publisher: | Bentham Science Publishers B.V. | Abstract: | Background: Eremurus spectabilis BIEB. (Liliaceae) is an edible and medicinal plant in Turkey. Introduction: This study was designed to isolate and quantify isoorientin in leaves of E. spectabilis and exhibit its effect on SH-SY5Y neuroblastoma cell line. Methods: Purity and identification of isoorientin were evaluated by 1D-NMR, 2D-NMR and Q-TOF were isolated from E. spectabilis via column chromatography. An HPLC method was also developed and validated for isoorientin. Results: Quantitative measurements indicated that contents of isoorientin in E. spectabilis leaves wereY 81.01 mg/g and MeOH extract were 23.75 mg/gr. All measurements were performed at 350 nm. Anticancer activity was investigated on cell culture. IC50 doses of isoorientin were detected as 250 µM at the 48th hour in SH-SY5Y cells by XTT assay. Real-time PCR analysis in SH-SY5Y cells showed that CCND1, CDK6, casp-9, Bax, ATR, Bcl-2, CHEK1 and CHEK2, expressions significantly reduced in experimental group when compared with the control group. p53, p21, caspase-3, caspase-8, Bcl-2, ATM and ERCC1 expressions increased in the experimental group when compared with the control group (P <0.05). Conclusion: Measurements revealed that E. spectabilis contains high amount of isoorientin and can be used as a new source of this compound. In addition to this, isoorientin affects cell proliferation of neuroblastoma cells by cell cycle control and apoptosis gene expression and it could be used as a therapeutic agent, however, more studies must be performed to clarify its mechanism. © 2018 Bentham Science Publishers. | URI: | https://hdl.handle.net/11499/11020 https://doi.org/10.2174/1573412913666170822162527 |
ISSN: | 1573-4129 |
Appears in Collections: | Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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