Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/23865
Full metadata record
DC FieldValueLanguage
dc.contributor.authorMete, Ergun-
dc.contributor.authorKaleli, I-
dc.contributor.authorCevahir, Nural-
dc.contributor.authorDemir, Melek-
dc.contributor.authorAkkaya, Yüksel-
dc.contributor.authorKiriş Satılmış, Özgün-
dc.date.accessioned2019-08-20T06:56:12Z-
dc.date.available2019-08-20T06:56:12Z-
dc.date.issued2017-
dc.identifier.issn0374-9096-
dc.identifier.urihttps://hdl.handle.net/11499/23865-
dc.identifier.urihttps://doi.org/10.5578/mb.53992-
dc.description.abstractEnterococci have recently become important due to their increased isolation rates in community-based and nosocomial infections and resistance to many antibiotics, including glycopeptides. The aim of this study was to evaluate the antimicrobial susceptible patterns and virulence factors of various clinical specimens; urine (n= 149), blood (n= 38), wound (n= 17), stool (n= 13), and other (n= 12) with a total of 229 enterococci including 138 E. faecalis and 91 E. faecium isolates. Aggregation factor (AF), enterococcus surface protein (esp), cytolysins and gelatinase encoding genes (asa1, esp, cylM, cylBcyl A, cylll, cylls, gelE, respectively) were investigated by molecular methods. Haemolysin production and gelatinase were studied phenotypically. A total of 30 isolates, 29 of E. faecium and one of E. faecalis isolates were resistant to vancomycin. High-level gentamicin and high-level streptomycin resistance in E. faecalis were 40.7% and 63.7% however, they were 47.1% and 55.8% in E. faecalis isolates. All strains were susceptible to linezolid. Ampicillin, penicillin and vancomycin resistance in E. faecium isolates were found to be higher than E. faecalis isolates (p= 0.001, p= 0.008 and p< 0.001). Asa1 (p< 0.001), cylll (p= 0.002) and cylls (p< 0.001) as well as gelatinase activity in isolates of E. faecalis were significantly higher than the isolates of E. faecium (p< 0.001). The most common virulence genes in our study were asa1 gene (45%), cyLs gene (33.2%) and esp gene (32.3%). Ciprofloxacin resistance in cylLL and cyLs gene positive isolates of E. faecalis were significantly higher compared to isolates that do not contain these genes (p= 0.035 and p= 0.047). Likewise, haemolysin producing E. faecium isolates were significantly more resistant to vancomycin compared to isolates that do not produce hemolysin (p< 0.001). When the virulence factors of vancomycin resistant and susceptible isolates were compared, the esp gene level in VRE E. faecium isolates was found to be 24.1%, while no esp gene was found in VRE E. faecalis isolates. The existence of asa1was negative in both VRE E. faecium and VRE E. faecalis isolates. The activity of hemolysin was found 42.3% for E. faecalis and 19.3% for E. faecium. In vancomycin-sensitive enterococcus (VSE) species, esp gene activity was 35.1% for E. faecalis, 29.4% for E. faecium, asa1 gene activity was 60.8% for E. faecalis and 47.1% for E. faecium, hemolysin activity was 52.8% for E. faecalis and 23.5% for E. faecium. In our study, it was found that VSE isolates have more virulence genes than VRE isolates. It should be kept in mind that VRE can causeinfections which are difficult-to-treat especially in hospitalized patients and VSE have significant virulence factors that can cause severe infections.en_US
dc.language.isotren_US
dc.publisherANKARA MICROBIOLOGY SOCen_US
dc.relation.ispartofMIKROBIYOLOJI BULTENIen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEnterococcus spp.; virulence factors; antimicrobial resistance;en_US
dc.subjectvancomycin resistant enterococcien_US
dc.titleEvaluation of virulence factors in enterococcus speciesen_US
dc.typeArticleen_US
dc.identifier.volume51en_US
dc.identifier.issue2en_US
dc.identifier.startpage101-
dc.identifier.startpage101en_US
dc.identifier.endpage114en_US
dc.authorid0000-0002-0854-2440-
dc.identifier.doi10.5578/mb.53992-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.pmid28566074en_US
dc.identifier.scopus2-s2.0-85021661739en_US
dc.identifier.wosWOS:000402308400001en_US
dc.identifier.scopusqualityQ3-
dc.ownerPamukkale University-
item.languageiso639-1tr-
item.fulltextWith Fulltext-
item.openairetypeArticle-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.dept14.03. Basic Medical Sciences-
crisitem.author.dept14.03. Basic Medical Sciences-
crisitem.author.dept14.03. Basic Medical Sciences-
crisitem.author.dept14.03. Basic Medical Sciences-
crisitem.author.dept14.03. Basic Medical Sciences-
crisitem.author.dept14.03. Basic Medical Sciences-
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
TR Dizin İndeksli Yayınlar Koleksiyonu / TR Dizin Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Files in This Item:
File SizeFormat 
virulence.pdf230.47 kBAdobe PDFView/Open
Show simple item record



CORE Recommender

SCOPUSTM   
Citations

12
checked on Nov 16, 2024

WEB OF SCIENCETM
Citations

9
checked on Nov 16, 2024

Page view(s)

110
checked on Aug 24, 2024

Download(s)

252
checked on Aug 24, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.