Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/24147
Title: MicroRNA-125b as a new potential biomarker on diagnosis of renal ischemia-reperfusion injury
Authors: Guclu, A
Kocak, C
Kocak, FE
Akcilar, R
Dodurga, Yavuz
Akcilar, A
Elmas, L
Keywords: Renal ischemia-reperfusion injury; Bardoxolone methyl; CDDO-Me;
MicroRNAs; miR-223-5p; miR-21; miR-125b
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
Abstract: Background: Acute renal failure is commonly seen in the perioperative period. Ischemia-reperfusion (IR) injury plays a major role in acute renal failure and delayed graft function. MicroRNAs (miRs), which are pivotal modulators of cell activities, offer a major opportunity for affective diagnosis and treatment strategies because they are tissue specific and in the center of gene expression modulation. The effect of bardoxolone methyl (BM) on miR-21, miR-223-5p, and miR-125b in renal IR injury was evaluated in this study.
Methods: Wistar-Albino rats (12-16 wk old, weighing 300-350 g) were used in the study. Rats (n = 6) were randomized into three groups (control, IR, and BM + IR). Tissue levels of miRs were analyzed with reverse transcription polymerase chain reaction.
Results: Significant reduction of urea and total oxidant status, increase of total antioxidant status, and oxidative stress index were identified in the IR + BM group compared with the IR group. Significant increases of miR-21 (2842.82-fold) and miR-125b (536.8-fold) were identified in the IR group compared with the control group; however, miR-223-5p levels did not show any significant difference. Also, miR-21 and miR-125b were significantly reduced in the IR + BM group compared with the IR group. Reduced histopathologic changes were observed in the IR + BM group. A significant decrease in the number of tunel-positive cells was identified in the IR + BM group compared with the IR group.
Conclusions: miR-125b was significantly increased in IR injury; thus, miR-125b can be a potential novel marker that can be used in diagnosis and treatment of renal IR injury. BM reduces miR-21 and miR-125b in case of IR injury and makes functional and histopathologic repairs. (C) 2016 Elsevier Inc. All rights reserved.
URI: https://hdl.handle.net/11499/24147
https://doi.org/10.1016/j.jss.2016.08.067
ISSN: 0022-4804
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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