The impacts of different auxins on phenolic contents and the total antioxidant capacity of hypericum retusum aucher

Abstract

The study was aimed to evaluate the effects of different auxins on phenolic contents and antioxidant potential of methanolic extract of Hypericum retusum Aucher (Clusiaceae) plantlets grown under in vitro conditions. After seed sterilisation and germination, shoot proliferations were performed. In this study, shoots were separately cultured in the medium containing BAP (0.5 mgl(-1)) combined with three different auxins (0.25 mgl(-1) IAA, NAA, IBA) and the highest number of shoots was obtained on medium supplemented with 0.5 mgl(-1) BAP. The highest total antioxidant capacity and phenolic contents were observed auxins. The methanol extracts of plantlets grown in vitro conditions showed the strongest free radical scavenging capacities at concentrations of 100 and 150 mu g/ml. It has been found that auxins and in vitro growth conditions have a significant effect on the total antioxidant capacity and phenolic contents.