Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/30085
Title: The sulfite molecule enhances homocysteine toxicity in SH-SY5Y cells
Authors: Gundogdu, G.
Dodurga, Yavuz
Küçükatay, Vural
Keywords: Homocysteine
Neurodegenerative diseases
Neurotoxicity
Sulfite
homocysteine
sulfite
antioxidant
sulfite oxidase
sulfur amino acid
Article
comet assay
controlled study
cytotoxicity
DNA damage
genotoxicity
human
human cell
in vitro study
nerve degeneration
neurotoxicity
oxidative stress
SH-SY5Y cell line
XTT assay
degenerative disease
drug effect
lipid peroxidation
metabolism
tumor cell line
Amino Acids, Sulfur
Antioxidants
Cell Line, Tumor
Comet Assay
DNA Damage
Humans
Lipid Peroxidation
Neurodegenerative Diseases
Oxidative Stress
Sulfite Oxidase
Sulfites
Publisher: Springer Netherlands
Abstract: Homocysteine (hcy) is an amino acid that contains sulfur species. In healthy individuals, plasma hcy levels are low. The aim of this study was to investigate the potential neurotoxic effects of hcy and sulfite (sft) molecules alone and in their combination, and also to identify the relationship of these substances on oxidative stress. SH-SY5Y cells were used as an invitro neurodegenerative disease model. The SH-SY5Y cells were treated with various concentrations of hcy alone, sft alone (final concentrations in the well were 10–250 µM and 0.1–5 mM, respectively) and a combination of both (hcy + sft). Their cytotoxicity and genotoxic effects were investigated using the XTT test and Comet assay and, their impact on oxidative stress was examined using total antioxidant–oxidant status (TAS-TOS) kits. The highest toxic doses of hcy and sft were found to be 250 µM and 5 mM, respectively, but the maximum toxic effect was observed for hcy + sft (p < 0.001). In addition, an increase in DNA damage was evident in all groups, but maximal damage was inflicted using in hcy + sft (p < 0.001). The oxidative stress index was significantly increased in hcy + sft (p < 0.05). Determining the increase in sft and hcy levels may contribute to delaying the occurrence of diseases before symptoms of neurodegenerative disease appear. © 2019, Springer Nature B.V.
URI: https://hdl.handle.net/11499/30085
https://doi.org/10.1007/s11033-019-04850-3
ISSN: 0301-4851
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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