Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/30110
Title: Genotypes and population genetics of cryptococcus neoformans and cryptococcus gattii species complexes in Europe and the mediterranean area
Authors: Cogliati, M.
Desnos-Ollivier, M.
McCormick-Smith, I.
Rickerts, V.
Ferreira-Paim, K.
Meyer, W.
Boekhout, T.
Keywords: Cryptococcus gattii
Cryptococcus neoformans
Europe
MLST
Population genetics
allele
Article
clonal variation
Cryptococcus neoformans var. grubii
Cryptococcus neoformans var. neoformans
fungal gene
fungus mutation
gene linkage disequilibrium
genetic analysis
genetic recombination
genetic variability
genotype
LAC1 gene
multilocus sequence typing
nonhuman
PLB1 gene
population genetics
population structure
priority journal
Southern Europe
animal
environmental microbiology
genetics
human
microbiological examination
phylogeny
Animals
Environmental Microbiology
Genetics, Population
Genotype
Humans
Mediterranean Region
Multilocus Sequence Typing
Mycological Typing Techniques
Phylogeny
Publisher: Academic Press Inc.
Abstract: A total of 476 European isolates (310 Cryptococcus neoformans var. grubii, 150 C. neoformans var. neoformans, and 16 C. gattii species complex) from both clinical and environmental sources were analyzed by multi-locus sequence typing. Phylogenetic and population genetic analyses were performed. Sequence analysis identified 74 sequence types among C. neoformans var. neoformans (VNIV), 65 among C. neoformans var. grubii (56 VNI, 8 VNII, 1 VNB), and 5 among the C. gattii species complex (4 VGI and 1 VGIV) isolates. ST23 was the most frequent genotype (22%) among VNI isolates which were mostly grouped in a large clonal cluster including 50% of isolates. Among VNIV isolates, a predominant genotype was not identified. A high percentage of autochthonous STs were identified in both VNI (71%) and VNIV (96%) group of isolates. The 16 European C. gattii species complex isolates analyzed in the present study originated all from the environment and all belonged to a large cluster endemic in the Mediterranean area. Population genetic analysis confirmed that VNI group of isolates were characterized by low variability and clonal expansion while VNIV by a higher variability and a number of recombination events. However, when VNI and VNIV environmental isolates were compared, they showed a similar population structure with a high percentage of shared mutations and the absence of fixed mutations. Also linkage disequilibrium analysis reveals differences between clinical and environmental isolates showing a key role of PLB1 allele combinations in host infection as well as the key role of LAC1 allele combinations for survival of the fungus in the environment. The present study shows that genetic comparison of clinical and environmental isolates represents a first step to understand the genetic characteristics that cause the shift of some genotypes from a saprophytic to a parasitic life style. © 2019 Elsevier Inc.
URI: https://hdl.handle.net/11499/30110
https://doi.org/10.1016/j.fgb.2019.04.001
ISSN: 1087-1845
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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