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https://hdl.handle.net/11499/30147
Title: | Caffeine prevents bilirubin-induced cytotoxicity in cultured newborn rat astrocytes | Authors: | Deliktaş, Mehmet Ergin, Hacer Demiray, Aydın Akça, Hakan Özdemir, Özmert M.A. Özdemir, Mehmet Bülent |
Keywords: | Bilirubin caffeine neurotoxicity newborn bilirubin catalase cytokine glutathione glutathione peroxidase interleukin 1beta interleukin 6 malonaldehyde nitrate nitrite superoxide dismutase toll like receptor toll like receptor 4 toll like receptor 9 tumor necrosis factor adenosine receptor blocking agent animal cell Article astrocyte astrocyte culture cell viability controlled study drug effect enzyme activity nervous system inflammation neuroapoptosis neuroprotection nonhuman priority journal rat animal cell culture complication hyperbilirubinemia preclinical study toxicity and intoxication Wistar rat Animals Animals, Newborn Astrocytes Caffeine Cells, Cultured Drug Evaluation, Preclinical Hyperbilirubinemia Neurotoxicity Syndromes Purinergic P1 Receptor Antagonists Rats, Wistar |
Publisher: | Taylor and Francis Ltd | Abstract: | Objective: Unconjugated bilirubin (UCB) may cause neurotoxicity in preterm neonates due to immaturity of UGT1A1 leading to bilirubin accumulation in the brain. Caffeine used in the treatment of apnea of prematurity was reported to decrease mechanical ventilation requirement, the frequencies of bronchopulmonary dysplasia, patent ductus arteriosus, cerebral palsy and neurodevelopmental disorders in very low birth weight infants. However, the effect of caffeine on hyperbilirubinemia was not yet clarified. Methods: We used astrocyte cell cultures obtained from 2-day-old Wistar albino rats via modified Cole and de Vellis method. UCB concentration toxic to 50% of astrocytes, and caffeine concentration increasing cell viability 100% were used in experiments. While no medication was applied to the control group, UCB (50 µM) and caffeine (100 µM) were applied to the bilirubin and caffeine groups for 24 h. Prophylactic and therapeutic caffeine groups were treated with caffeine 4 h before and after UCB exposure. The effects of caffeine were investigated in rat astrocytes exposed to UCB in terms of cell viability, apoptosis, antioxidant defense, proinflammatory cytokines, and Toll-like receptor (TLR)s. Results: Compared to the control group, UCB increased apoptosis, malondialdehyde (MDA), tumor necrosis factor-? (TNF-?), interleukin (IL)-1ß, IL-6, total nitrate/nitrite, and TLR4 levels, and decreased cell viability, catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) activities, glutathione, and TLR9 levels (for all p <.001). Conversely, prophylactic and therapeutic caffeine improved the detrimental effects of UCB. Conclusions: Caffeine seems encouraging for the prevention and treatment of bilirubin neurotoxicity in rats by means of its antiapoptotic, antioxidant, anti-inflammatory, anti-nitrosative, and anti-TLR-4 properties. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group. | URI: | https://hdl.handle.net/11499/30147 https://doi.org/10.1080/14767058.2017.1419175 |
ISSN: | 1476-7058 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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