Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/30189
Title: Data collection on marine litter ingestion in sea turtles and thresholds for good environmental status
Authors: Matiddi, M.
DeLucia, G.A.
Silvestri, C.
Darmon, G.
Tomás, J.
Pham, C.K.
Camedda, A.
Vandeperre, Frederic
Claro, Françoise
Kaska, Yakup
Kaberi, Helen
Revuelta, Ohiana
Piermarini, Raffaella
Daffina, Roberto
Pisapia, Marco
Genta, Daniela
Sözbilen, Doğan
Bradai, Mohamed N.
Rodríguez, Yasmina
Gambaiani, Delphine
Tsangaris, Catherine
Chaieb, Olfa
Moussier, Judicaëlle
Loza, Ana L.
Miaud, Claude
Keywords: EcAp process
Environmental sciences
Good environmental status
Issue 147
Marine litter
MSFD
Necropsy
Plastic ingestion
Sea turtle
Thresholds
animal
eating
ecosystem
environmental monitoring
gastrointestinal tract
information processing
physiology
turtle
Animals
Data Collection
Eating
Ecosystem
Environmental Monitoring
Gastrointestinal Tract
Turtles
Publisher: Journal of Visualized Experiments
Abstract: The following protocol is intended to respond to the requirements set by the European Union’s Marine Strategy Framework Directives (MSFD) for the D10C3 Criteria reported in the Commission Decision (EU), related to the amount of litter ingested by marine animals. Standardized methodologies for extracting litter items ingested from dead sea turtles along with guidelines on data analysis are provided. The protocol starts with the collection of dead sea turtles and classification of samples according to the decomposition status. Turtle necropsy must be performed in authorized centers and the protocol described here explains the best procedure for gastrointestinal (GI) tract isolation. The three parts of the GI (esophagus, stomach, intestine) should be separated, opened lengthways and contents filtered using a 1 mm mesh sieve. The article describes the classification and quantification of ingested litter, classifying GI contents into seven different categories of marine litter and two categories of natural remains. The quantity of ingested litter should be reported as total dry mass (weight in grams, with two decimal places) and abundance (number of items). The protocol proposes two possible scenarios to achieve the Good Environmental Status (GES). First: “There should be less than X% of sea turtles having Y g or more plastic in the GI in samples of 50-100 dead turtles from each sub-region”, where Y is the average weight of plastic ingested and X% is the percentage of sea turtles with more weight (in grams) of plastic than Y. The second one, which considers the food remain versus plastic as a proxy of individual health, is: “There should be less than X% of sea turtles having more weight of plastic (in grams) than food remains in the GI in samples of 50-100 dead turtles from each sub-region”. © 2019 Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
URI: https://hdl.handle.net/11499/30189
https://doi.org/10.3791/59466
ISSN: 1940-087X
Appears in Collections:Acıpayam Meslek Yüksekokulu Koleksiyonu
Fen-Edebiyat Fakültesi Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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