Phytochemical analysis, phenolic content, antioxidant, antibacterial, insecticidal and cytotoxic activites of Allium reuterianum Boiss. Extracts

Abstract

Dried bulbs and leaves of Allium reuterianum were extracted with different solvents and evaluated for antioxidant, antibacterial, insecticidal, in vitro cytotoxic activities. The obtained results indicated that the highest compound 3, 4-dihidroksi benzoic acid with 166.2 µg/g in extracts. The ethanolic extracts had the highest phenolic content and acetone extracts had the lowest fenolic content. In the ß-carotene-linoleic acid test system, bulb methanol (ABM) and leaf methanol (ALM) extracts showed the highest antioxidant activity. The mean antioxidant activity of ABM and ALM were 75.76±1.22% and 73.42±1.03%, respectively. The highest ferric-reducing power of extract (ABM) was determined 12.75±0.010 trolox equivalent (mg/g). Methanolic extract (ALM) exhibited a dose-dependent scavenging of DPPH and ABTS radicals with IC50 values of 0.512±0.003 mg/mL and 0.378±0.002 mg/mL respectively. Very strong reduction of gram-positive Staphylococcus aureus growth were observed during incubation of bacteria in bulb extracts (MIC was 20±1.01 µg/mL). The brine shrimp lethality assay of bulb extract has showed good toxic to brine shrimp nauplii, with LC50 of 3.987 µg/mL. The bulb extract of A. reuterianum showed highest larvicidal activity against Cx. pipiens with value LC50 (6.4129 µg/mL). The results suggest that these plants could be used as a source of natural antioxidant and antibacterial agents. © 2019, National Institute of Science Communication and Information Resources (NISCAIR). All rights reserved.