LARVICIDAL EFFECT OF PINUS BRUTIA TEN. SEED ESSENTIAL OIL TO PINE

Abstract

Thaumetopoea wilkinsoni Tams. is one of the most widespread pest found in the pine forest of Turkey. The objective of this study was to determine larvicidal effects of essential oil obtained from Pinus brutia Ten. on the third and fourth instar larvae of T. wilkinsoni under laboratory conditions. In the present study, seed essential oil was tested at seven different doses (5 to 1000 ppm) against to this pest. The chemical composition of the oil was determined by GC-MS. The essential oil of P. brutia was dominated by monoterpenes. Totally, 14 chemical compounds were identified by GC-MS analysis. beta-Pinene (38.15%) and alpha-pinene (24.99%) were identified as major chemical components followed by trans-beta-caryophyllene (15.24 %) and germacrene-D (4.25 %). As a result of analysis, P. brutia seed oil showed high larvicidal activity, with LD50 values of 187.88, 122.83 and 68.77 ppm, LD90 values of 608.85, 479.31 and 300.16 ppm at 24, 48 and 72 hours, respectively. The results showed that the activities were dose dependent. Therefore, this essential oil could be useful for to find management strategies against populations of T. wilkinsoni. In this study, the larvicidal activity of essential oil from P. brutia against T. wilkinsoni was evaluated for the first time.

C1 [Semiz, Gurkan; Kocabiyik, Kubra] Pamukkale Univ, Fac Arts & Sci, Dept Biol, Denizli, Turkey.

[Cetin, Huseyin] Akdeniz Univ, Dept Biol, Fac Sci, Antalya, Turkey.