Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/37123
Title: Adipose derived mesenchymal stem cell treatment in experimental asherman syndrome induced rats
Authors: Çil, Nazlı
Yaka, Mutlu
Ünal, Murat Serkant
Dodurga, Yavuz
Tan, Semih
Seçme, Mücahit
Karagür, Ege Rıza
Keywords: Asherman syndrome
IGF-1
Mesenchymal stem cells
miR-199a
miR-98
VEGF
broxuridine
eosin
estrogen
formaldehyde
hematoxylin
microRNA
microRNA 199a
microRNA 98
somatomedin C
unclassified drug
vasculotropin
adipose derived stem cell
animal experiment
animal model
animal tissue
Article
controlled study
endometrium
female
fibrosis
follow up
gene expression
immunohistochemistry
inflammation
mesenchymal stem cell transplantation
nonhuman
protein expression
rat
uterus horn
uterus synechia
Western blotting
Publisher: Springer
Abstract: Asherman syndrome (AS) occurs due to fibrosis or uterine adhesions as a result of damage to the basal layer of the endometrium. The aim of this study is investigating the effects of adipose tissue-derived mesenchymal stem cell (ADMSC) application on the expression of vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF-1), miRNA-98, miRNA199a in endometrial tissue in rats with AS. Study groups were designed as, control (C), Asherman syndrome (AS), AS + oral estrogen (ASO), AS + ADMSC (ASSC), AS + oral estrogen + ADMSC (ASSCO) with 7 samples in each group. Characterization and differentiation experiments were performed in ADMSC obtained. Two weeks after the development of the AS, ADMSC therapy was applied. BrdU (5-bromo-2'-deoxyuridine) labeling was performed to show the presence of ADMSC in the tissues. Rats were sacrificed after 8 weeks and bilateral uterine horn resection was performed. Tissues were fixed in formaldehyde. After routine tissue follow-up, sections were taken and evaluated with hematoxylin eosin staining. VEGF1 and IGF1 expressions were evaluated by immunohistochemical staining and western blot analysis. Expression changes of miR-98 and miR-199a were detected by RT-PCR. Our results showed that stem cells and estrogen giving together reduced inflammation and fibrosis in the endometrium. Immunohistochemistry and western blot results suggested that this effect was achieved especially through IGF-1. In our study, decreased miR-98 and miR-199a expressions were determined in Asherman syndrome. Furthermore, no changes of miRNA expressions were observed in treatment groups. © 2020, Springer Nature B.V.
URI: https://hdl.handle.net/11499/37123
https://doi.org/10.1007/s11033-020-05505-4
ISSN: 0301-4851
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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