Antioxidant and phenolic characterization with HPLC of various extract of verbascum glomeratum linneus

Abstract

Verbascum L. species have been used since ancient times in traditional medicine thanks to their bioactive compounds. In this study, we evaluated the antioxidant activity and phenolic contents of ethanolic and acetonic extract of Verbascum glomeratum that collected from Denizli, Turkey. The antioxidant activity were evaluated by using various methods (ß-carotene/linoleic acid, ferric reducing power assays (FRAP), radical scavenging assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2'-azino-bis-3-ethyl benzthiazoline-6-sulphonic acid (ABTS), phoshomolibdenum methods) and total phenolic, flavonoid and tannin contents in the extracts were determined. Antioxidant activity was determined as ß-carotene/linoleic acid (%58.5±0.021), DPPH (IC50: 1.220±0.025) and ABTS (IC50:1.552±0.021), ferric reducing antioxidant power (FRAP) (4.151±0.056 mgTE/g), phoshomolibdenum assay (8.218±0.015 mgAE/g) total phenolic, total flavonoid and tannin contents was found as (4.41±0.007 mgGAE/mL), (87.142±0.009 µQE/g), and (30.178±0.008 mgCEs/g) respectively, and then 14 different phenolic compounds in ethanol extracts was carried out by HPLC. Epicatechin (2742.09 µg/g) and 2,5dihidroksi (2544.96 µg/g) were the most abundant phenolic constituents in the extracts. According the results, the extract of V. glomeratum may be considered as a potential source of biological agents. © 2020 Chemical Society of Pakistan. All rights reserved.