Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/37191
Title: Protective effects of vitamin E on aluminium sulphate-induced testicular damage
Authors: Ulfanov, O.
Cil, N.
Adıgüzel, Esat
Keywords: Aluminium
apoptosis
testis
TUNEL
vitamin E
alpha tocopherol
aluminum sulfate
eosin
hematoxylin
animal cell
animal experiment
animal model
animal tissue
Article
cell count
cell structure
controlled study
drug effect
epithelium cell
germ layer
Johnsen score
male
male genital tract parameters
nonhuman
rat
scoring system
semen analysis
seminiferous tubule epithelium
sham procedure
spermatozoon count
testis injury
testis tissue
tissue structure
TUNEL assay
Wistar rat
Publisher: SAGE Publications Ltd
Abstract: Male infertility can be caused by environmental factors, genetic defects, physiological and endocrine deficiencies and testicular pathologies. Aluminium (Al) can cause male infertility through a number of mechanisms. The aim of our study was thus to determine whether vitamin E (VitE) has protective effects on Al-induced testicular damage, which was determined according to sperm counts and morphology and using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method. Thirty-four male Wistar rats (250–300 g) were randomly assigned to control (no procedures performed; n = 6) or 0.2 mL intraperitoneal injection group (n = 7 each; three times per week for 4 weeks): sham (distilled water), 10 mg/kg Al, 500 mg/kg VitE and 10 mg/kg Al plus 500 mg/kg VitE (Al + VitE). Sperm samples were evaluated for andrological parameters. The testes were examined by haematoxylin/eosin. The epithelial thickness and areas were calculated and Johnsen scores were determined for the germinal epithelium; the apoptotic indices were determined from TUNEL staining. For Al, the bonds between the germinal epithelial cells were broken in some tubules, and there were unidentified cells in the lumen of some tubules. For control, sham and VitE, normal morphology of the germinal epithelium was generally preserved. With Al + VitE, the full germinal epithelium cell series was maintained, with only mature sperm in the lumen. TUNEL-positive cells were significantly higher with Al compared to control and sham (p < 0.05). For Al + VitE, the number of apoptotic cells was reduced compared to Al alone and was therefore similar to control, sham and VitE (p > 0.05). Our findings show that Al caused testicular damage. VitE reduced the number of apoptotic cells during the damage caused by Al. © The Author(s) 2020.
URI: https://hdl.handle.net/11499/37191
https://doi.org/10.1177/0748233720919663
ISSN: 0748-2337
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Show full item record



CORE Recommender

SCOPUSTM   
Citations

7
checked on Oct 13, 2024

WEB OF SCIENCETM
Citations

6
checked on Dec 20, 2024

Page view(s)

44
checked on Aug 24, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.