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https://hdl.handle.net/11499/37201
Title: | Assessment of the protective and therapeutic effect of melatonin against thioacetamide-induced acute liver damage | Authors: | Sayan, M. Karabulut, D. Özdamar, Saim |
Keywords: | caspase-3 LC3 melatonin RIP3 thioacetamide alanine aminotransferase alkaline phosphatase aspartate aminotransferase caspase 3 protein protein lc3 protein rip3 transforming growth factor transforming growth factor beta tumor necrosis factor unclassified drug antioxidant lymphotoxin microtubule associated protein receptor interacting protein serine threonine kinase receptor-interacting protein 3, rat alanine aminotransferase blood level alkaline phosphatase blood level animal experiment animal model animal tissue apoptosis Article aspartate aminotransferase blood level autophagy (cellular) controlled study drug effect drug mechanism immunohistochemistry liver histology liver protection male nonhuman rat single drug dose thioacetamide-induced liver injury acute disease animal blood liver metabolism oxidative stress pathology toxic hepatitis Wistar rat Acute Disease Alanine Transaminase Alkaline Phosphatase Animals Antioxidants Aspartate Aminotransferases Caspase 3 Chemical and Drug Induced Liver Injury Immunohistochemistry Liver Lymphotoxin-alpha Male Melatonin Microtubule-Associated Proteins Oxidative Stress Rats Rats, Wistar Receptor-Interacting Protein Serine-Threonine Kinases Thioacetamide Tumor Necrosis Factor-alpha |
Publisher: | John Wiley and Sons Inc. | Abstract: | Acute or chronic damage to the liver may occur through alcohol, drugs, viruses, genetic disorders, and toxicity. In this study, we planned to investigate the protective and therapeutic effects of melatonin (Mel) by causing damage to the liver with thioacetamide (TAA). Thirty-five rats were used. Group I: control group (seven pieces), group II: Mel group (seven pieces) the single dose on the first day of the experiment was 10 mg/kg, group III: TAA (seven pieces) 300 mg/kg with 24-hour intervals, two doses, group IV: Mel + TAA group (seven pieces) 10 mg/kg single dose Mel was applied 24 hours before TAA application, group V: TAA + Mel group (seven pieces) single dose (24th hour) of 10 mg/kg Mel was administered after TAA (300 mg/kg) two doses. The liver histology was evaluated. Apoptosis, autophagy, and necrosis markers in tissue were determined by immunohistochemistry. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) levels in blood serum samples and transforming growth factor-ß (TGF-ß) and tumor necrosis factor-? (TNF-?) levels were determined in liver tissue. TAA affected histologically the classical lobule structure both in cell cords and sinusoids. Caspase-3, RIP3, and LC3 levels were increased in group III compared with the control group. TAA did not cause a statistically significant change in TNF-? level but decreased the TGF-ß level significantly. AST and ALT levels were statistically significant in group II and V compared with group I, the ALP level was significant in group IV compared with group II. The results of this study showed that TAA caused significant damage to tissues and increased cell death, Mel was found to have more therapeutic than the protective effect on tissues. © 2020 Wiley Periodicals, Inc. | URI: | https://hdl.handle.net/11499/37201 https://doi.org/10.1002/jbt.22450 |
ISSN: | 1095-6670 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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