Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/37201
Title: Assessment of the protective and therapeutic effect of melatonin against thioacetamide-induced acute liver damage
Authors: Sayan, M.
Karabulut, D.
Özdamar, Saim
Keywords: caspase-3
LC3
melatonin
RIP3
thioacetamide
alanine aminotransferase
alkaline phosphatase
aspartate aminotransferase
caspase 3
protein
protein lc3
protein rip3
transforming growth factor
transforming growth factor beta
tumor necrosis factor
unclassified drug
antioxidant
lymphotoxin
microtubule associated protein
receptor interacting protein serine threonine kinase
receptor-interacting protein 3, rat
alanine aminotransferase blood level
alkaline phosphatase blood level
animal experiment
animal model
animal tissue
apoptosis
Article
aspartate aminotransferase blood level
autophagy (cellular)
controlled study
drug effect
drug mechanism
immunohistochemistry
liver histology
liver protection
male
nonhuman
rat
single drug dose
thioacetamide-induced liver injury
acute disease
animal
blood
liver
metabolism
oxidative stress
pathology
toxic hepatitis
Wistar rat
Acute Disease
Alanine Transaminase
Alkaline Phosphatase
Animals
Antioxidants
Aspartate Aminotransferases
Caspase 3
Chemical and Drug Induced Liver Injury
Immunohistochemistry
Liver
Lymphotoxin-alpha
Male
Melatonin
Microtubule-Associated Proteins
Oxidative Stress
Rats
Rats, Wistar
Receptor-Interacting Protein Serine-Threonine Kinases
Thioacetamide
Tumor Necrosis Factor-alpha
Publisher: John Wiley and Sons Inc.
Abstract: Acute or chronic damage to the liver may occur through alcohol, drugs, viruses, genetic disorders, and toxicity. In this study, we planned to investigate the protective and therapeutic effects of melatonin (Mel) by causing damage to the liver with thioacetamide (TAA). Thirty-five rats were used. Group I: control group (seven pieces), group II: Mel group (seven pieces) the single dose on the first day of the experiment was 10 mg/kg, group III: TAA (seven pieces) 300 mg/kg with 24-hour intervals, two doses, group IV: Mel + TAA group (seven pieces) 10 mg/kg single dose Mel was applied 24 hours before TAA application, group V: TAA + Mel group (seven pieces) single dose (24th hour) of 10 mg/kg Mel was administered after TAA (300 mg/kg) two doses. The liver histology was evaluated. Apoptosis, autophagy, and necrosis markers in tissue were determined by immunohistochemistry. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) levels in blood serum samples and transforming growth factor-ß (TGF-ß) and tumor necrosis factor-? (TNF-?) levels were determined in liver tissue. TAA affected histologically the classical lobule structure both in cell cords and sinusoids. Caspase-3, RIP3, and LC3 levels were increased in group III compared with the control group. TAA did not cause a statistically significant change in TNF-? level but decreased the TGF-ß level significantly. AST and ALT levels were statistically significant in group II and V compared with group I, the ALP level was significant in group IV compared with group II. The results of this study showed that TAA caused significant damage to tissues and increased cell death, Mel was found to have more therapeutic than the protective effect on tissues. © 2020 Wiley Periodicals, Inc.
URI: https://hdl.handle.net/11499/37201
https://doi.org/10.1002/jbt.22450
ISSN: 1095-6670
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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