Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/37419
Title: Expression and DNA methylation profiles of EZH2-target genes in plasma exosomes and matched primary tumor tissues of the patients with diffuse large B-cell lymphoma
Authors: Barış, İkbal Cansu
Hacıoğlu, Sibel
Türk, Nilay Şen
Çetin, Gökhan Ozan
Zencır, S.
Bağcı, Gülseren
Caner, Vildan
Keywords: Diffuse large B-cell lymphoma
Epigenetics
Exosome
EZH2-target genes
Publisher: Springer Science and Business Media Deutschland GmbH
Abstract: Aims: Diffuse large B-cell lymphoma (DLBCL) is the most common type of aggressive lymphoma. This study was designed to compare epigenetic alterations observed in Enhancer of Zeste Homolog 2 (EZH2)-target genes between plasma-derived exosomes and primary tumors in DLBCL patients. Main methods: Exosomes were isolated from plasma of 21 DLBCL patients and 21 controls. We analyzed the methylation status of the target genes using methylation-specific PCR. We also examined whether the exosomes and the tumor samples contained transcripts of the target genes. Key findings: We found that CDKN2A and CDKN2B were methylated in both plasma exosomes and primary tumor tissue samples. None of the transcripts were found in the exosomes except CDKN1B which was expressed in 8 (38%) of the exosome samples. Significance: This study showed that plasma exosomes might preferably package certain target molecules from primary tumors and the exosomes containing dual methylated DNAs of CDKN2A and CDKN2B, or CDKN1B transcript may contribute to DLBCL pathogenesis. © 2020, Federación de Sociedades Españolas de Oncología (FESEO).
URI: https://hdl.handle.net/11499/37419
https://doi.org/10.1007/s12094-020-02504-6
ISSN: 1699-048X
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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