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https://hdl.handle.net/11499/37419
Title: | Expression and DNA methylation profiles of EZH2-target genes in plasma exosomes and matched primary tumor tissues of the patients with diffuse large B-cell lymphoma | Authors: | Barış, İkbal Cansu Hacıoğlu, Sibel Türk, Nilay Şen Çetin, Gökhan Ozan Zencır, S. Bağcı, Gülseren Caner, Vildan |
Keywords: | Diffuse large B-cell lymphoma Epigenetics Exosome EZH2-target genes |
Publisher: | Springer Science and Business Media Deutschland GmbH | Abstract: | Aims: Diffuse large B-cell lymphoma (DLBCL) is the most common type of aggressive lymphoma. This study was designed to compare epigenetic alterations observed in Enhancer of Zeste Homolog 2 (EZH2)-target genes between plasma-derived exosomes and primary tumors in DLBCL patients. Main methods: Exosomes were isolated from plasma of 21 DLBCL patients and 21 controls. We analyzed the methylation status of the target genes using methylation-specific PCR. We also examined whether the exosomes and the tumor samples contained transcripts of the target genes. Key findings: We found that CDKN2A and CDKN2B were methylated in both plasma exosomes and primary tumor tissue samples. None of the transcripts were found in the exosomes except CDKN1B which was expressed in 8 (38%) of the exosome samples. Significance: This study showed that plasma exosomes might preferably package certain target molecules from primary tumors and the exosomes containing dual methylated DNAs of CDKN2A and CDKN2B, or CDKN1B transcript may contribute to DLBCL pathogenesis. © 2020, Federación de Sociedades Españolas de Oncología (FESEO). | URI: | https://hdl.handle.net/11499/37419 https://doi.org/10.1007/s12094-020-02504-6 |
ISSN: | 1699-048X |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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