Cyclamen l. Inhibits nitric oxide production in lps-stimulated nsclc cells

Abstract

Cyclamen L., belonging to the Primulaceae family, is a tuberous perennial geophyte with some taxa indigenous to Turkey. However, this genus has been poorly investigated for its cytotoxic and anticancer potentials. The current study aimed to explore the antiproliferative effects of the ethanolic extracts of three Cyclamen taxa (C. pseudibericum, C. mirabile and C. persicum) and their nitric oxide (NO) inhibitory activity in LPS-stimulated non-small cell lung cancer (NSCLC) cell lines, namely HCC78 and H1975. Also, total saponin contents of the extracts were determined as quillaja equivalents. The cytotoxicity of the Cyclamen extracts was assessed by the CellTiter-Glo assay. C. persicum extract caused a higher cytotoxic effect on both H1975 and HCC78 cells than the other two Cyclamen extracts and its IC50 values in H1975 and HCC78 cells were determined to be 17.27 and 34.15 ?g/ mL, respectively. While Griess reaction was performed to determine the nitrite levels as an index of NO production in LPS-stimulated NSCLC cells treated with the Cyclamen extracts, vanillin-sulphuric acid method was used to detect total saponin contents in the extracts. Among the three Cyclamen taxa evaluated, the highest inhibitory activity towards NO production in HCC78 cells was obtained with from C. pseudibericum, while C. persicum showed the highest inhibitory activity in H1975 cells. As a result, this study demonstrated that the tuber extracts of three Cyclamen taxa, which have been determined their total saponin contents, had significant cytotoxic activity and NO inhibitory potentials against HCC78 and H1975 non-small cell lung cancer cell lines. These data suggest that Cyclamen L. extracts examined in this study merit further research so as to isolate the bioactive secondary metabolites with anti-tumor potentials. © 2020 DSR Publishers/The University of Jordan. All Rights Reserved.