Effect of Different Doses of Vitamin E with Selenium on Renal Damage Caused by Unilateral Ureteral Obstruction Model?

Abstract

Objective: Chronic kidney disease (CKD) is still common worldwide. We investigated the effect of two different doses of vitamin E with selenium (Se) administration to prevent CKD leads to renal damage via unilateral ureteral obstruction (UUO).

Material and Methods: Thirty-two female Wistar Albino rats were divided into four groups; a sham group (left UUO+no medication); left UUO with no treatment, left UUO treated with 100 mg/kg dose Vitamin E+Se (RPVE+Se) and left UUO treated with 1000 mg/kg Vitamin E+Se (HDVE+Se) mixture. All rats subjected during 14 days and killed humanely. Malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), levels were determined in kidney tissue, whereas total antioxidant status ( TAS), total oxidant status (TOS) and DNA damage marker 8-hydroxy-2-deoxyguanosine (8-OHdG) in serum. Histologic examination were also done.

Results: The unilateral ureteral obstruction model increased MDA (p<0.05), TOS (p<0.001) and 8-OHdG levels (p<0.001) via oxidative mechanisms. In the treatment groups increased TAS, SOD (p< 0.001), and GSH (p<0.001) levels were determined, but in RPVE+Se group, TAS levels were slightly higher than the HDVE+Se group (p=0,039). In terms of histological findings, tubular necrosis (p=0.003) and lymphocyte infiltration (p=0.002) were observed renal tissue images. RPVE+Se group findings were similar to the sham group, while in HDVE group these images similar to the UUO group.

Conclusions: It was observed that UUO caused oxidative stress resulting in renal damage, and controlled vitamin E application with Selenium administration, which is considered to be an effective antioxidant couple, inhibited oxidative stress.