Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/3783
Title: A novel functional conducting polymer as an immobilization platform
Authors: Ak, Metin
Guler, Emine
Soyleyici, Hakan Can
Demirkol, Dilek Odaci
Timur, Suna
Keywords: Electrochemistry
Conducting polymers
Spectroscopic analysis
Amperometric
Amperometry
Analytical characterization
Biosensing
G. oxydans
Gluconobacter oxydans
Glucose concentration
Microbial biosensor
Pyranose oxidase
Electropolymerization
Publisher: Elsevier B.V.
Abstract: Here, we present the fabrication of conducting polymer based enzymatic and microbial biosensors. To obtain immobilization platforms for both pyranose oxidase (PyOx) and Gluconobacter oxydans, the graphite electrode surface was modified with the polymer of 4-amino-N-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl) benzamide (HKCN) which has free amino groups on the surface for further bioconjugation reactions with the biomolecules. Initially, the electrode surface was covered with HKCN via electropolymerization. Then, either PyOx or G. oxydans cell was stabilized using glutaraldehyde as a cross-linker. After optimization of biosensors, analytical characterization and surface imaging studies were investigated. The change of current depends on glucose concentration between 0.05-1.0 mM and 0.25-2.5 mM with HKCN/PyOx and HKCN/G. oxydans biosensors in batch systems. Also, the calibration graphs were obtained for glucose in FIA mode, and in this case, linear ranges were found to be 0.01-1.0 mM and 0.1-7.5 mM for HKCN/PyOx and HKCN/G. oxydans, respectively.
URI: https://hdl.handle.net/11499/3783
ISSN: 0928-4931
Appears in Collections:Fen-Edebiyat Fakültesi Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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