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https://hdl.handle.net/11499/3919
Title: | Enhancing biosensor properties of conducting polymers via copolymerization: Synthesis of EDOT-substituted bis(2-pyridylimino)isoindolato-palladium complex and electrochemical sensing of glucose by its copolymerized film | Authors: | Tekbasoglu, Tugce Yazici Soganci, Tugba Ak, Metin Koca, Atif Sener, Muhammet Kasim |
Keywords: | Electrochemistry Conducting polymers Spectroscopic analysis Synthesis Biosensors Copolymerization Electrodes Electropolymerization Modified electrode Enzyme immobilization Enzymes Glucose Glucose sensors Mass spectrometry Palladium Palladium compounds 3 ,4-ethylenedioxythiophene Bioelectrocatalytic reactions Electrochemical methods Electrochemical sensing Glucose oxidase Functionalized monomers Coordination compounds Metal complex Indol derivatives |
Publisher: | Elsevier B.V. | Abstract: | 1,3-Bis(2-pyridylimino)isoindoline derivative bearing 3,4-ethylenedioxythiophene (EDOT-BPI) and its palladium complex (EDOT-PdBPI) were synthesized and characterized by FT-IR, 1H NMR, 13C NMR, UV–Vis spectroscopies and via mass spectrometric analysis. Polymerization of EDOT-PdBPI and copolymerization with 4-amino-N-(2,5-di(thiophene-2-yl)-1H-pyrrol-1-yl)benzamide (HKCN) were carried out by an electrochemical method. In addition, P(EDOT-PdBPI-co-HKCN) modified graphite rod electrode was improved for amperometric glucose sensor based on glucose oxidase (GOx). In this novel biosensor matrix, amino groups in HKCN were used for the enzyme immobilization. On the other hand, EDOT-PdBPI used to mediate the bioelectrocatalytic reaction. Amperometric detection was carried out following oxygen consumption at -0.7 V vs. the Ag reference electrode in phosphate buffer (50 mM, pH 6.0). The novel biosensor showed a linear amperometric response for glucose within a concentration range of 0.25 mM to 2.5 mM (LOD: 0.176 mM). Amperometric signals at 1 mM of glucose were 17.9 µA under anaerobic conditions. Amperometric response of the P(EDOT-PdBPI-co-HKCN)/GOx electrode decreased only by 13% within eight weeks. The P(EDOT-PdBPI-co-HKCN)/GOx electrode showed good selectivity in the presence of ethanol and phenol. This result shows that, modification of the proposed biosensor by copolymerization of amine functionalized monomer, which is indispensable to the enzyme immobilization, with palladium complex bearing monomer, which is mediate the bioelectrocatalytic reaction, have provided to give perfect response to different glucose concentrations. | URI: | https://hdl.handle.net/11499/3919 | ISSN: | 0956-5663 |
Appears in Collections: | Fen-Edebiyat Fakültesi Koleksiyonu PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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