Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/3919
Title: Enhancing biosensor properties of conducting polymers via copolymerization: Synthesis of EDOT-substituted bis(2-pyridylimino)isoindolato-palladium complex and electrochemical sensing of glucose by its copolymerized film
Authors: Tekbasoglu, Tugce Yazici
Soganci, Tugba
Ak, Metin
Koca, Atif
Sener, Muhammet Kasim
Keywords: Electrochemistry
Conducting polymers
Spectroscopic analysis
Synthesis
Biosensors
Copolymerization
Electrodes
Electropolymerization
Modified electrode
Enzyme immobilization
Enzymes
Glucose
Glucose sensors
Mass spectrometry
Palladium
Palladium compounds
3 ,4-ethylenedioxythiophene
Bioelectrocatalytic reactions
Electrochemical methods
Electrochemical sensing
Glucose oxidase
Functionalized monomers
Coordination compounds
Metal complex
Indol derivatives
Issue Date: 15-Jan-2017
Publisher: Elsevier B.V.
Abstract: 1,3-Bis(2-pyridylimino)isoindoline derivative bearing 3,4-ethylenedioxythiophene (EDOT-BPI) and its palladium complex (EDOT-PdBPI) were synthesized and characterized by FT-IR, 1H NMR, 13C NMR, UV–Vis spectroscopies and via mass spectrometric analysis. Polymerization of EDOT-PdBPI and copolymerization with 4-amino-N-(2,5-di(thiophene-2-yl)-1H-pyrrol-1-yl)benzamide (HKCN) were carried out by an electrochemical method. In addition, P(EDOT-PdBPI-co-HKCN) modified graphite rod electrode was improved for amperometric glucose sensor based on glucose oxidase (GOx). In this novel biosensor matrix, amino groups in HKCN were used for the enzyme immobilization. On the other hand, EDOT-PdBPI used to mediate the bioelectrocatalytic reaction. Amperometric detection was carried out following oxygen consumption at -0.7 V vs. the Ag reference electrode in phosphate buffer (50 mM, pH 6.0). The novel biosensor showed a linear amperometric response for glucose within a concentration range of 0.25 mM to 2.5 mM (LOD: 0.176 mM). Amperometric signals at 1 mM of glucose were 17.9 µA under anaerobic conditions. Amperometric response of the P(EDOT-PdBPI-co-HKCN)/GOx electrode decreased only by 13% within eight weeks. The P(EDOT-PdBPI-co-HKCN)/GOx electrode showed good selectivity in the presence of ethanol and phenol. This result shows that, modification of the proposed biosensor by copolymerization of amine functionalized monomer, which is indispensable to the enzyme immobilization, with palladium complex bearing monomer, which is mediate the bioelectrocatalytic reaction, have provided to give perfect response to different glucose concentrations.
URI: https://hdl.handle.net/11499/3919
ISSN: 0956-5663
Appears in Collections:Fen-Edebiyat Fakültesi Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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