Use of super-structural conducting polymer as functional immobilization matrix in biosensor design

Abstract

The evolution of electrochemical biosensors reflects a simplification and enhancement of the transduction pathway. The use of novel conducting polymers in the preparation of sensor platforms has become increasingly studied and imparts many advantages. The sensitivity and overall performance of enzymatic biosensors has improved tremendously as a result of incorporating functional group containing conducting polymers into their fabrication. Hereby, an efficient surface design was investigated by modifying the graphite rod electrode surfaces with conducting polymer displaying functional groups for the immobilization of biomolecules. A model enzyme, glucose oxidase, was efficiently immobilized to the modified surfaces via covalent binding. The biosensor was characterized in terms of its storage and operational stability and kinetic parameters. The designed sensor platform revealed excellent stability and promising kinetic parameters without carbon nanotube or graphene additive. Finally, the sensor platform was tested on beverages for glucose detection.