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https://hdl.handle.net/11499/4117
Title: | Effects of metals and detergents on biotransformation and detoxification enzymes of leaping mullet (Liza saliens) | Authors: | Şen, Alaattin. Semiz, Aslı. |
Keywords: | 7-Ethoxyresorufin O-deethylase Biomarkers Detergents Glutathione S-transferases Inhibition Liza saliens Metals Mullet 1 chloro 2,4 dinitrobenzene aluminum antimony cadmium cobalt copper cytochrome P450 1A detergent etacrynic acid ethoxyresorufin ethoxyresorufin deethylase ferrous ion glutathione glutathione transferase microsome enzyme nickel zinc biomarker biotransformation enzyme activity metal PAH perciform pollution effect aquatic environment aquatic species article conjugation controlled study detoxification enzyme inhibition enzyme substrate in vitro study liver liver microsome nonhuman statistical significance Animals Biological Markers Biotransformation Cytochrome P-450 CYP1A1 Dinitrochlorobenzene Environmental Monitoring Enzyme Inhibitors Ethacrynic Acid Fish Proteins Glutathione Transferase Liver Metabolic Detoxication, Drug Microsomes, Liver Oxazines Smegmamorpha Substrate Specificity Water Pollutants, Chemical |
Abstract: | In this study, feral leaping mullet (Liza saliens) liver microsomal 7-ethoxyresorufin O-deethylase (EROD), and cytosolic glutathione S-transferases (GSTs) activities were investigated using 7-ethoxyresorufin, 1-chloro-2,4-dinitrobenzene (CDNB), and ethacrynic acid (EA) as substrates, respectively. The average EROD activity was found as 1139±175 pmol resorufin/min/mg protein. The average GST activities towards CDNB and EA were found as 1364±41 and 140±19 nmol/min/mg protein, respectively. We have, then, investigated the in vitro effects of some metals and detergents on CYP1A and GST activities in leaping mullet liver. Leaping mullet liver microsomal EROD activity was significantly inhibited by Hg (0.1 mM), Ni (0.1 mM), Cd (0.1 mM), Cu (0.1 mM), Zn (0.1 mM), Sb (0.1 mM), Fe2+ (1 mM), Co (1 mM), Al (1 mM), and Fe3+ (1 mM), with the percent inhibition of 80, 80, 77, 75, 70, 69, 56, 53, 46, and 44, respectively. Similarly, conjugation of CDNB catalyzed by GST was inhibited significantly to lesser extend by Hg (0.1 mM), Sb (0.1 mM), Cd (0.1 mM), Cu (0.1 mM), Zn (0.1 mM), Fe3+ (1 mM), Co (1 mM), and Fe2+ (1 mM), with the percent inhibition of 70, 69, 65, 61, 54, 51, 47, and 43, respectively. The degrees of inhibition observed on GST catalyzed EA conjugation by Hg (0.1 mM), Cd (0.1 mM), Sb (0.1 mM), Cu (0.1 mM), and Zn (0.1 mM) were 86, 78, 69, 51, and 42, respectively. In addition to metals, the effect of various detergents on leaping mullet liver EROD, GST-CDNB, and GST-EA activities were studied. It was found that ionic detergents strongly inhibited the EROD activity, whereas much less inhibitions were observed with GST catalyzed activities. Therefore, the CYP1A inhibition potencies of metals and detergents suggest that their contribution to the overall CYP1A induction in polycyclic aromatic hydrocarbons contaminated environmental samples has to be taken into account for better interpretation of environmental studies. © 2006 Elsevier Inc. All rights reserved. | URI: | https://hdl.handle.net/11499/4117 https://doi.org/10.1016/j.ecoenv.2006.08.007 |
ISSN: | 0147-6513 |
Appears in Collections: | Fen-Edebiyat Fakültesi Koleksiyonu PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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