Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/4319
Title: Tempol prevents harmful effects of remote ischemia reperfusion injury on healing of experimental colonic anastomoses
Authors: Aydın, Hüseyin Çağatay.
Teke, Z.
Aytekin, Faruk Önder.
Yenisey, Çiğdem.
Kabay, Burhan.
Simsek, N.G.
Tekin, K.
Keywords: Colonic anastomosis
Ischemia-reperfusion
Tempol
glutathione
hydroxyproline
malonaldehyde
myeloperoxidase
tempol
animal experiment
animal model
animal tissue
article
colon anastomosis
colon ischemia
controlled study
enzyme activity
healing
in vivo study
male
nonhuman
postoperative period
priority journal
rat
reperfusion injury
statistical significance
superior mesenteric artery
Anastomosis, Surgical
Animals
Antioxidants
Colon
Constriction
Cyclic N-Oxides
Disease Models, Animal
Male
Mesenteric Artery, Superior
Rats
Rats, Wistar
Reperfusion Injury
Spin Labels
Wound Healing
Abstract: Background and aims: Tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine- N-oxyl) is a water-soluble analogue of the spin label TEMPO. As an antioxidative agent, it is a member of nitroxides, which detoxifies superoxide and possibly other toxic radicals in vivo. In this study, we aimed to investigate whether tempol prevents harmful systemic effects of superior mesenteric ischemia-reperfusion on left colonic anastomosis in rats. Materials and methods: Anastomosis of the left colon was performed in 30 rats that were divided into three groups each having ten animals: sham-operated control (group I), 60 min of intestinal ischemia-reperfusion by superior mesenteric artery occlusion (group II), and tempol-treated group (30 mg/kg before and after the ischemia-reperfusion (group III). On postoperative day 5, all animals were killed and anastomotic bursting pressures were measured in vivo. Tissue samples were obtained for further investigation of anastomotic hydroxyproline content, perianastomotic malondialdehyde, and glutathione levels. Results: There was a statistically significant increase in the quantity of myeloperoxidase activity and malondialdehyde levels in group II, along with a decrease in glutathione levels, anastomotic hydroxyproline content, and bursting pressure values when compared to controls. However, all of the investigated parameters were normalized in tempol-treated animals (group III). Conclusion: We conclude that tempol significantly prevents harmful systemic effects of reperfusion injury on colonic anastomoses in a rat model of superior mesenteric artery occlusion. © Springer-Verlag 2006.
URI: https://hdl.handle.net/11499/4319
https://doi.org/10.1007/s00384-006-0149-y
ISSN: 0179-1958
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Show full item record



CORE Recommender

SCOPUSTM   
Citations

12
checked on Sep 30, 2024

WEB OF SCIENCETM
Citations

14
checked on Sep 30, 2024

Page view(s)

38
checked on Aug 24, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.