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https://hdl.handle.net/11499/4393
Title: | Investigation of asymptomatic visceral leishmaniasis cases using western blot in an endemic area in Turkey | Authors: | Sakru, N. Korkmaz, M. Ozbel, Y. Ertabaklar, H. Şengül, Mustafa. Toz, S.O. |
Keywords: | Asymptomatic Diagnosis Leishmaniasis Turkey Western blot parasite antigen protozoon antibody adolescent adult aged animal blood child endemic disease enzyme linked immunosorbent assay evaluation female fluorescent antibody technique human immunology infant isolation and purification Leishmania infantum male methodology middle aged newborn parasitology pathophysiology preschool child review Turkey (republic) visceral leishmaniasis Western blotting Adolescent Adult Aged Animals Antibodies, Protozoan Antigens, Protozoan Blotting, Western Child Child, Preschool Endemic Diseases Enzyme-Linked Immunosorbent Assay Female Fluorescent Antibody Technique, Indirect Humans Infant Infant, Newborn Leishmaniasis, Visceral Male Middle Aged |
Abstract: | In Turkey, Leishmania infantum is responsible for human visceral leishmaniasis (VL), which is seen mainly in the Aegean, Mediterranean, and Central Anatolia Regions. This study aimed to determine asymptomatic infections in an endemic area of VL in Turkey using the western blot technique. A total of 82 persons including children and adults were chosen randomly in Denizli province which is one of the endemic sites for VL. Serum samples were collected and screened using indirect immunofluorescent test (IFAT), enzyme-linked immunosorbent assay (ELISA) and western blot (WB). One year later, 35 of the 82 persons were sampled and screened serologically for the second time. Seven out of 82 samples were found to be positive by western blot analysis with the presence of 14 and/or 18 kDa bands. Two of these seven sera were also positive by IFAT, but only one of these two was positive by ELISA. Only one person showing seropositivity with all three tests had clinical symptoms and was diagnosed as VL with the presence of amastigotes in bone marrow aspirate. Because six people, including the one found to be seropositive in all two tests, had no clinical symptoms, they were accepted as asymptomatic carriers. The ratio of asymptomatic infection was calculated as 7.41% (6/81) in the region. In the second sampling, the western blot revealed antibodies against the same antigens in all seven subjects. Our findings showed that the presence of antibodies against 14 and 18 kDa antigens are important for the diagnosis of symptomatic and asymptomatic infections. Western blot was found to be effective in the detection of asymptomatic persons in the epidemiological studies in endemic areas. | URI: | https://hdl.handle.net/11499/4393 | ISSN: | 1121-7138 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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