Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/4410
Title: The protective effects of omega - 3 fatty acids against MK-801-induced neurotoxicity in prefrontal cortex of rat
Authors: Özyurt, Bülent.
Sarsilmaz, M.
Akpolat, N.
Ozyurt, H.
Akyol, O.
Herken, Hasan.
Kus, I.
Keywords: Apoptosis
Malondialdehyde
MK-801
Omega - 3 fatty acids
Oxidative stress
Prefrontal cortex
Protein carbonyl
adenosine deaminase
carbonyl derivative
catalase
dizocilpine
docosahexaenoic acid
glutathione peroxidase
icosapentaenoic acid
malonaldehyde
marincap
nitric oxide
omega 3 fatty acid
superoxide dismutase
xanthine oxidase
animal cell
animal experiment
animal model
animal tissue
apoptosis
article
controlled study
decapitation
diet supplementation
drug mechanism
enzyme activity
experimental animal
histology
lipid peroxidation
male
microscopy
neurochemistry
neuropathology
neurophysiology
neuroprotection
neurotoxicity
nonhuman
oxidation kinetics
oxidative stress
prefrontal cortex
priority journal
prognosis
psychosis
rat
rat strain
schizophrenia
statistical significance
Animals
Dizocilpine Maleate
Male
Rats
Abstract: The aims of this study are to investigate the contribution effect of oxidative stress in MK-801-induced experimental psychosis model, and to show that prevention of oxidative stress may improve prognosis. Because oxidative damage has been suggested in the neuropathophysiology of schizophrenia, the possible protecting agents against lipid peroxidation are potential target for the studies in this field. For this purpose, Wistar Albino rats were divided into three groups: the first group was used as control, MK-801 was given to the rats in the second group and MK-801 + ? - 3 essential fatty acids (EFA) was given to the third group. MK-801 was given intraperitoneally at the dose of 0.5 mg/(kg day) once a day for 5 days in experimental psychosis group. In the second group, 0.8 g/(kg day), ? - 3 FA (eicosapentaenoic acid, 18%, docosahexaenoic acid, 12%) was given to the rats while exposed MK-801. In control group, saline was given intraperitoneally at the same time. After 7 days, rats were killed by decapitation. Prefrontal brain area was removed for histological and biochemical analyses. As a result, malondialdehyde (MDA), as an indicator of lipid peroxidation, protein carbonyl (PC), as an indicator of protein oxidation, nitric oxide (NO) levels and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities as antioxidant enzymes, and xanthine oxidase (XO) and adenosine deaminase (AD) activities as an indicator of DNA oxidation was found to be increased significantly in prefrontal cortex (PFC) of MK-801 group (P < 0.0001) compared to control group. In ? - 3 FA treated rats, prefrontal tissue MDA, PC and NO levels as well as SOD, GSH-Px, XO, and AD enzyme activities were significantly decreased when compared to MK-801 groups (P < 0.0001) whereas catalase (CAT) enzyme activity was not changed. Moreover, in the light of microscopic examination of MK-801 groups, a great number of apoptotic cells were observed. ? - 3 FA supplementation decreased the apoptotic cell count in PFC. The results of this study revealed that oxidative stress and apoptotic changes in PFC may play an important role in the pathogenesis of MK-801-induced neuronal toxicity. This experimental study also provides some evidences for the protective effects of ? - 3 FA on MK-801-induced changes in PFC of rats. © 2006.
URI: https://hdl.handle.net/11499/4410
https://doi.org/10.1016/j.neuint.2006.08.002
ISSN: 0197-0186
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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