Evaluation of thiol-disulphide homeostasis in patients with chronic hepatitis B

Abstract

Aim: To evaluate the relationship between dynamic thiol-disulfide homeostasis which is a novel oxidative stress marker and levels of HBV DNA, and the oxidant-antioxidant balance.Material and Method: In the controlled study which included chronic hepatitis B (CHB) patients and healthy volunteers, dynamic Thiol-disulphide homeostasis (TDH) was measured using a novel automated method developed by Erel. Disulfide / total thiol (%), disulfide / native thiol (%), and native thiol / total thiol (%) rates were calculated using the previously determined concentrations of disulfides, native thiols, and total thiols.Results: Of thiol / disulfide homeostasis parameters, native thiol, total thiol, and disulfide levels were statistically lower in the CHB patient group (p <0.05). As a result of the correlation analyses, a significant negative correlation was determined between HBV DNA levels and disulfide / native thiol, disulfide / total thiol, and native thiol / total thiol parameters (p <0.05).Conclusions: Our results suggest that oxidative stress increases with the rise in HBV-DNA levels and that the antioxidant defense may have weakened.