Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray

Abstract

Objective: To investigate the regenerative effects of adipose-derived mesenchymal stem cells (ADMSCs) in immortalized human keratinocyte cells (HaCaT) exposed to ultraviolet B (UVB) rays. Materials and Methods: Epidermal cells were interacted with UVB rays. Then, treatment was applied to ADMSC mediums prepared in different concentrations. Cells that did not receive UVB light were included in the study as a control. Results: With the application of human adipose tissue mesenchymal stem cells to HaCaT cells with different concentrations of media prepared, cell viability increased in both cells that received UVB radiation and those that did not. It has been observed that the said increases are more pronounced, especially in cells that have received UVB rays, reaching the maximum value at 100%, which is the highest stem cell medium concentration. ADMSC medium also had an increasing effect on the migration of UVB (+) cells. In UVB (+) cells, decreased levels of myeloid cell leukemia 1 protein (Mc1-1) and phosphorylated extracellular signal-regulated kinases (p-ERK 1/2) and dose-proportional increases were observed after treatment with stem cell medium. Conclusion: It has been determined that some factors in the ADMSC medium, especially in keratinocytes that have received UVB rays, positively support proliferation and show reparative and rejuvenating functions.