Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/46235
Title: Dose-dependent effects of adalimumab in neonatal rats with hypoxia/reoxygenation-induced intestinal damage
Authors: Kocamaz, Halil
Ozdemir, Ozmert
Turk, Nilay Sen
Enli, Yasar
Sahin, Barbaros
Ergin, Hacer
Keywords: Adalimumab
hypoxia
intestine
reperfusion
tumor necrosis factor-alpha
Necrotizing Enterocolitis
Antioxidant Capacity
Lipid-Peroxidation
Acute-Pancreatitis
Oxidative Stress
Infliximab
Ischemia
Injury
Publisher: Assoc Basic Medical Sci Federation Bosnia & Herzegovina Sarajevo
Abstract: Tumor necrosis factor-alpha (TNF-alpha) has an important role in hypoxia/reoxygenation (H/R)-induced intestinal damage. It was shown that blocking TNF-alpha with infliximab has beneficial effects on experimental necrotizing enterocolitis and hypoxic intestinal injury. However, there is no data about the effect of adalimumab on H/R-induced intestinal damage. Therefore, we aimed to determine potential dose-dependent benefits of adalimumab in such damage in neonatal rats. Wistar albino rat pups were assigned to one of the four groups: control group, hypoxia group, low-dose adalimumab (5 mg/kg/day) treated group (LDAT), and high-dose adalimumab (50 mg/kg/day) treated group (HDAT). On the fourth day of the experiment, all rats except for the control group were exposed to H/R followed by euthanasia. Malondialdehyde (MDA), myeloperoxidase (MPO), TNF-alpha, total antioxidant capacity (TAC), and total oxidant capacity (TOC) were measured in intestinal tissue. TAC and TOC values were used to calculate the oxidative stress index (OSI). Histopathological injury scores (HIS) were also evaluated in the tissue samples. MDA levels were significantly lower in the LDAT and HDAT groups (p < TNF-alpha levels were significantly lower in the LDAT group (p < 0.001). OSI was significantly higher in the H/R group than in the control and LDAT groups (p < 0.001). Mean HIS values in the LDAT group were significantly lower than those in the H/R and HDAT groups (p < 0.001). This experimental study showed that low-dose adalimumab appears to have a beneficial effect on intestinal injury induced with H/R in neonatal rats.
URI: https://doi.org/10.17305/bjbms.2020.4823
https://hdl.handle.net/11499/46235
ISSN: 1512-8601
1840-4812
Appears in Collections:Hayvan Islahı ve Genetik Uygulama ve Araştırma Merkezi Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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