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https://hdl.handle.net/11499/4646
Title: | Zoledronic acid is synergic with vinblastine to induce apoptosis in a multidrug resistance protein-1 dependent way: An in vitro study | Authors: | Yıldız, Mustafa Celik-Ozenci, C. Akan, S. Akan, I. Sati, L. Demir, R. Savas, B. |
Keywords: | Apoptosis Multidrug resistance TUNEL Vinblastine Zoledronic acid multidrug resistance protein 1 vinblastine zoledronic acid apoptosis article cytotoxicity dose response drug combination drug inhibition drug mechanism drug potentiation human human cell in vitro study nick end labeling Diphosphonates Drug Synergism Humans Imidazoles Immunoblotting P-Glycoprotein |
Abstract: | We have explored the action of zoledronic acid, which has an apoptotic effect and is used as an agent for treating skeletal metastases and osteoporosis, in the presence of vinblastine, and whether this effect is associated with MRP-1 (multidrug resistance protein-1) expression. HEK (human embryonic kidney) 293 cells were transfected to form the multidrug resistant cell line designated 293MRP (MRP-1 expressing HEK293 cells). Both lines were treated with varying concentrations of vinblastine and zoledronic acid. Apoptosis was determined by the TUNEL (deoxyuridine triphosphate nick end-labeling) method. The type of treatment, MRP-1 expression status, and the type of treatment with respect to MRP-1 expression status significantly affected (P < 0.001) the degree of apoptosis. The largest increase in cytotoxicity was noted in HEK293 cells, when 100 µmol zoledronic acid was added to 4 µg/ml vinblastine (an increment of 80.3%, P < 0.001). This preliminary work shows that zoledronic acid acts synergistically with vinblastine to induce apoptosis in an MRP-1 dependent way. © 2005 Published by Elsevier Ltd on behalf of International Federation for Cell Biology. | URI: | https://hdl.handle.net/11499/4646 https://doi.org/10.1016/j.cellbi.2005.11.008 |
ISSN: | 1065-6995 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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