Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/4646
Title: Zoledronic acid is synergic with vinblastine to induce apoptosis in a multidrug resistance protein-1 dependent way: An in vitro study
Authors: Yıldız, Mustafa
Celik-Ozenci, C.
Akan, S.
Akan, I.
Sati, L.
Demir, R.
Savas, B.
Keywords: Apoptosis
Multidrug resistance
TUNEL
Vinblastine
Zoledronic acid
multidrug resistance protein 1
vinblastine
zoledronic acid
apoptosis
article
cytotoxicity
dose response
drug combination
drug inhibition
drug mechanism
drug potentiation
human
human cell
in vitro study
nick end labeling
Diphosphonates
Drug Synergism
Humans
Imidazoles
Immunoblotting
P-Glycoprotein
Abstract: We have explored the action of zoledronic acid, which has an apoptotic effect and is used as an agent for treating skeletal metastases and osteoporosis, in the presence of vinblastine, and whether this effect is associated with MRP-1 (multidrug resistance protein-1) expression. HEK (human embryonic kidney) 293 cells were transfected to form the multidrug resistant cell line designated 293MRP (MRP-1 expressing HEK293 cells). Both lines were treated with varying concentrations of vinblastine and zoledronic acid. Apoptosis was determined by the TUNEL (deoxyuridine triphosphate nick end-labeling) method. The type of treatment, MRP-1 expression status, and the type of treatment with respect to MRP-1 expression status significantly affected (P < 0.001) the degree of apoptosis. The largest increase in cytotoxicity was noted in HEK293 cells, when 100 µmol zoledronic acid was added to 4 µg/ml vinblastine (an increment of 80.3%, P < 0.001). This preliminary work shows that zoledronic acid acts synergistically with vinblastine to induce apoptosis in an MRP-1 dependent way. © 2005 Published by Elsevier Ltd on behalf of International Federation for Cell Biology.
URI: https://hdl.handle.net/11499/4646
https://doi.org/10.1016/j.cellbi.2005.11.008
ISSN: 1065-6995
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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