Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/46610
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dc.contributor.authorSaglam, Tugba-
dc.contributor.authorDusen, Serdar-
dc.contributor.authorMete, Ergun-
dc.contributor.authorKaraman, Ulku-
dc.date.accessioned2023-01-09T21:15:30Z-
dc.date.available2023-01-09T21:15:30Z-
dc.date.issued2022-
dc.identifier.issn1230-2821-
dc.identifier.issn1896-1851-
dc.identifier.urihttps://doi.org/10.1007/s11686-021-00494-1-
dc.identifier.urihttps://hdl.handle.net/11499/46610-
dc.description.abstractPurpose While Toxoplasma gondii (T. gondii) infection is asymptomatic in immunocompetent individuals, it is a life-threatening protozoan in immunocompromised individuals. Its water-borne transmission to humans poses a serious public health concern. Polymerase Chain Reaction (PCR) has a considerable potential for the sensitive and specific detection of T. gondii oocysts in waters. Methods Comparative evaluation of RE 529-bp sequence and B1 gene to detect T. gondii tachyzoites and oocysts via PCR in agricultural irrigation water taken from downtown Denizli, Turkey and water samples collected from neighborhood fountains was performed for the first time in Turkish context. Results Based on real-time PCR targeting the B1 genetic markers and RE 529-bp sequence, T. gondii DNA was identified in 6 (16.7%) out of 48 samples collected from agricultural irrigation water. Besides, our PCR analysis did not establish any presence of T. gondii in drinking water samples. Conclusion T. gondii showed lower sensitivity in B1-based PCR than in PCR targeting RE 529-bp sequence.en_US
dc.description.sponsorshipPamukkale University Scientific Research Projects Uniten_US
dc.description.sponsorshipPamukkale University Scientific Research Projects Unit.en_US
dc.language.isoenen_US
dc.publisherSpringer Int Publ Agen_US
dc.relation.ispartofActa Parasitologicaen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectToxoplasma gondiien_US
dc.subject529-bpen_US
dc.subjectB1 geneen_US
dc.subjectWater-borneen_US
dc.subjectDenizlien_US
dc.subjectTurkeyen_US
dc.subjectTime Pcren_US
dc.subjectOocystsen_US
dc.subjectCryptosporidiumen_US
dc.subjectPurificationen_US
dc.subjectInfectionen_US
dc.titleComparative Evaluation of Re 529-Bp Sequence and B1 Gene in the Detection of Toxoplasma gondii Through PCR in Water Samples of Denizli, Turkeyen_US
dc.typeArticleen_US
dc.identifier.volume67en_US
dc.identifier.issue1en_US
dc.identifier.startpage555en_US
dc.identifier.endpage559en_US
dc.authoridSAĞLAM GÜVENDİK, Tuğba/0000-0003-1654-2261-
dc.identifier.doi10.1007/s11686-021-00494-1-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.authorscopusid57351211800-
dc.authorscopusid6602783160-
dc.authorscopusid23498029300-
dc.authorscopusid13611422300-
dc.authorwosidSAĞLAM GÜVENDİK, Tuğba/HIZ-7076-2022-
dc.identifier.pmid34817741en_US
dc.identifier.scopus2-s2.0-85120638550en_US
dc.identifier.wosWOS:000722115300001en_US
dc.identifier.scopusqualityQ3-
item.languageiso639-1en-
item.fulltextWith Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.grantfulltextopen-
crisitem.author.dept17.02. Biology-
crisitem.author.dept14.03. Basic Medical Sciences-
Appears in Collections:Fen-Edebiyat Fakültesi Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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