Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/47364
Title: Electrochemical ?-fetoprotein immunosensor based on Fe3O4NPs@covalent organic framework decorated gold nanoparticles and magnetic nanoparticles including SiO2@TiO2
Authors: Bölükbaşi Ö.S.
Yola B.B.
Karaman C.
Atar, Necip
Yola M.L.
Keywords: Immunosensor
Nanocomposite
Voltammetry
?-Fetoprotein
alpha fetoprotein
gold
magnetite nanoparticle
metal organic framework
silicon dioxide
titanium
titanium dioxide
chemistry
genetic procedures
human
immunoassay
procedures
alpha-Fetoproteins
Biosensing Techniques
Gold
Humans
Immunoassay
Magnetite Nanoparticles
Metal-Organic Frameworks
Silicon Dioxide
Titanium
Publisher: Springer
Abstract: The early diagnosis of major diseases such as cancer is typically a major issue for humanity. Human ?-fetoprotein (AFP) as a sialylated glycoprotein is of approximately 68 kD molecular weight and is considered to be a key biomarker, and an increase in its level indicates the presence of liver, testicular, or gastric cancer. In this study, an electrochemical AFP immunosensor based on Fe3O4NPs@covalent organic framework decorated gold nanoparticles (Fe3O4 NPs@COF/AuNPs) for the electrode platform and double-coated magnetic nanoparticles (MNPs) based on SiO2@TiO2 (MNPs@SiO2@TiO2) nanocomposites for the signal amplification was fabricated. The immobilization of anti-AFP capture antibody was successfully performed on Fe3O4 NPs@COF/AuNPs modified electrode surface by amino-gold affinity, while the conjugation of anti-AFP secondary antibody on MNPs@SiO2@TiO2 was achieved by the electrostatic/ionic interactions. Transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) analysis, cyclic voltammetry (CV), square wave voltammetry (SWV), and electrochemical impedance spectroscopy (EIS) techniques were used to characterize the nanostructures in terms of physical and electrochemical features. The limit of detection (LOD) was 3.30 fg mL?1. The findings revealed that the proposed electrochemical AFP immunosensor can be effectively used to diagnose cancer. Graphical abstract: [Figure not available: see fulltext.] © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
URI: https://doi.org/10.1007/s00604-022-05344-z
https://hdl.handle.net/11499/47364
ISSN: 0026-3672
Appears in Collections:Mühendislik Fakültesi Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Show full item record



CORE Recommender

SCOPUSTM   
Citations

29
checked on Oct 13, 2024

WEB OF SCIENCETM
Citations

34
checked on Oct 31, 2024

Page view(s)

56
checked on Aug 24, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.