Biological activity and phytochemical characterisation of potentilla Davisii R. R Mill & h duman

Abstract

Potentilla species belong the family Rosaceae which have been used in precious medicinal, phytochemical and pharmacological studies. In current study, we aimed to determined antioxidant capacity, total secondary metabolites and phenolic composition of acetone and methanol extracts of Potentilla davisii. To evaluate antioxidant activity of extracts of P. davisii was used different five methods (P-carotene-linoleic acid assay, DPPH and ABTS radical scavenging activity, Phosphomolybdenum assay, CUPRAC assay). Total phenolic, flavonoid and tannin content were determined using Folin-Ciocalteau colorimetric method, Aliminum chlorid method and Sulphiric acid-vanillin method separately. Characterisation of methanol extract of P. davisii was analysed by HPLC method with 15 standards. The results showed that acetone extracts have highest total antioxidant activity, while methanol extracts have the highest radical scavenging activity and reducing power activity. Also, acetone extracts possess more total flavonoid and tannin amount. HPLC analysis indicated that caffeic acids is found as most common phenolic acid in methanol extract of P. davisii. According to these results, extracts of P. davisii could be natural product for medicinal, biological and industrial studies. © 2021 Parlar Scientific Publications. All rights reserved.