Insertion / Deletion Polymorphism Of Angiotensin-converting Enzyme Gene In Ovarian Hyperstimulation Syndrome Patients

Abstract

Purpose: Previous studies have shown that ovary contains all the components of renin-angiotensin system. Renin-angiotensin system may play a primary role in the pathogenesis of ovarian hyperstimulation syndrome (OHSS). In this study our purpose was to investigate the relationship between the angiotensin-converting enzyme (ACE) gene insertion / deletion polymorphism and OHSS, in high risk patients for the syndrome. Materials and methods: 47 patients who have undergone controlled ovarian hyperstimulation cycle (control n: 25; OHSS n: 22) were recruited in the study. Estradiol levels of all patients in both group were higher than 3000 pg/ml . Age, duration of infertility, total gonadotropin dose, induction time, total follicular number, endometrial thickness and cause of infertility were recorded. DNA was extracted, and the insertion/deletion polymorphism of ACE gene was detected by polymerase chain reaction (PCR). Results: There were no statistically difference in age, duration of infertility, total gonadotropin dose, induction time and endometrial thickness between control and OHSS group. Follicle number was higher in OHSS group (p<0.001). There were no difference in the ACE II - ID - DD genotypes between OHSS and control group patients (p>0.05). Homozygotes for the deletion polymorphism (DD genotype) were found 1.47 times higher in OHSS group (OR: 1.47 %95 confidence interval, 0.44 - 4.86) but this was statistically insignificant. Conclusion: These data indicate that ACE gene I/D polymorphism is not associated with OHSS. ACE gene polymorphism doesn't play a major role in the pathogenesis of OHSS. © 2019, Pamukkale University. All rights reserved.