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https://hdl.handle.net/11499/4864
Title: | Does melatonin protect or treat brain damage from traumatic oxidative stress? | Authors: | Kerman, M. Cirak, B. Ozguner, M.F. Dagtekin, A. Sutcu, R. Altuntas, I. Delibas, N. |
Keywords: | Antioxidant enzymes Experimental brain damage Lipid peroxidation Melatonin catalase glutathione glutathione peroxidase malonaldehyde melatonin superoxide dismutase animal model animal tissue antioxidant activity article brain contusion brain injury controlled study craniotomy drug mechanism dura mater enzyme activity enzyme analysis lipid peroxidation male neuroprotection nonhuman oxidative stress priority journal Animals Antioxidants Brain Brain Injuries Catalase Free Radical Scavengers Glutathione Peroxidase Lipid Peroxidation Male Oxidative Stress Rabbits Superoxide Dismutase |
Abstract: | A variety of experimental studies have demonstrated the neuroprotective effects of melatonin, based on its antioxidant activity. In a prospective randomized study, the effects of melatonin were investigated in experimental head trauma-induced oxidative stress in rabbits. The experimental study was performed on 30 rabbits. The animals were divided into three groups. Group I (sham procedure): a right parietal craniotomy was performed on each animal, and the dura mater was left intact. Group II: experimental brain trauma (EBT) was performed on each animal using a 1 cm inner diameter × 10 cm long glass tube, through which a 20 g weight (0.5 cm diameter) was dropped onto the brain at the craniotomy site, causing a contusional head trauma. Group III: the same EBT model was performed, but 2.5 mg/kg melatonin was injected intraperitoneally four times (total dose 10 mg/kg); these injections were performed 20 min before the operation, during the trauma, 1 h later and 2 h later. The rabbits were sacrificed after the EBT at 24 h after the brain trauma. The activities of the three principal antioxidant enzymes-catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px)-were determined, and the levels of malondialdehyde (MDA), a product of lipid peroxidation, and glutathione (GSH) were measured in brain homogenates. MDA levels were found to be higher in the EBT group than in the EBT+melatonin group or the sham procedure group. The SOD activity was found to be higher in the EBT group than in the sham procedure group. Enzymatic parameters (except for SOD) were significantly higher in melatonin-treated animals than in EBT animals. GSH levels in melatonin-treated animals were decreased compared with EBT animals. In conclusion, the data indicate that melatonin protects against free radical-mediated oxidative changes in brain tissue by boosting antioxidant enzymes, and in particular lowering lipid peroxidation in rabbits with EBT. © Springer-Verlag 2005. | URI: | https://hdl.handle.net/11499/4864 https://doi.org/10.1007/s00221-005-2338-2 |
ISSN: | 0014-4819 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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