Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/4874
Title: Protective effects of antithrombin III on retinal ischemia/reperfusion injury in rats: A histopathologic study
Authors: Özden, S.
Müftüoglu, S.
Tatlıpınar, Sinan.
Kaymaz, F.
Yıldırım, Cem.
Yaylalı, Volkan.
Özbay, D.
Keywords: Antithrombin III
Apoptosis
Ischemia/reperfusion injury
Retinal ischemia
Thrombin
antithrombin III
animal experiment
animal model
animal tissue
apoptosis
article
cell death
cell infiltration
controlled study
electron microscopy
histopathology
mononuclear cell
nonhuman
priority journal
rat
reperfusion injury
retina
retina ischemia
vertebral artery
Animals
Cell Count
Disease Models, Animal
Ischemia
Leukocytes
Microscopy, Electron
Rats
Rats, Wistar
Reperfusion Injury
Retinal Diseases
Retinal Vessels
Serine Proteinase Inhibitors
Abstract: PURPOSE. To investigate the effect of antithrombin III (AT III) on retinal ischemia/reperfusion (I/R) injury in rats. METHODS. The study was carried out on 10 Wistar albino rats (20 eyes) and four-vessel occlusion method was employed to induce retinal ischemia in this study. Rats were divided into two groups: Group I (control group, 10 eyes) and Group II (AT III, 10 eyes). In both groups, vertebral arteries were occluded bilaterally an electric needle coagulator under an operating microscope. A total of 48 hours after the initial procedure, the rats were re-anesthetized and both common carotid arteries were clamped to interrupt blood flow. In Group II, rats were injected intravenously with 250 U/kg of AT III 5 minutes before the induction of ischemia. Duration of ischemia was 30 minutes. At the end of this period, clamp was removed for the reperfusion of the eye for 4 hours. Following the reperfusion period, the animals were killed by decapitation. Retinal sections were evaluated under light and electron microscope. The signs of I/R injury at the microscopic level, i.e., cellular degeneration, vacuolization between retinal layers, increase in the retinal thickness due to edema, mononuclear cell infiltration, and apoptotic cells, were recorded for each group. RESULTS. Retinal sections obtained from the rats in the AT III group revealed a well preserved retinal structure. When average thickness values of the two groups were compared to each other, the difference was significant with respect to inner nuclear and inner plexiform layers indicating increased retinal thickness values in Group I due to tissue edema resulting from I/R injury. Similarly, mononuclear cell infiltration and apoptotic cell counts were found to be significantly higher in control group compared to AT III group showing the inhibitory effect of AT III on leukocyte infiltration and apoptotic cell death in rat retina. CONCLUSIONS. Antithrombin III attenuated I/R injury in rat retina. © Wichtig Editore, 2005.
URI: https://hdl.handle.net/11499/4874
ISSN: 1120-6721
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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