Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/4907
Title: Mycobacterium tuberculosis complex DNA does not exist in atheromatous plaques
Authors: Rota, Simin
Tuncer, Serdar
Kanat, O.
Keywords: Atherosclerosis
M. tuberculosis
PCR
Phospholipid
bacterial DNA
BCG vaccine
chloroform
phenol
primer DNA
proteinase K
adult
article
atherosclerotic plaque
clinical article
controlled study
coronary artery atherosclerosis
coronary artery bypass graft
coronary artery disease
electrophoresis
endarterectomy
extraction
female
human
human tissue
male
Mycobacterium tuberculosis
polymerase chain reaction
precipitation
tissue section
Coronary Arteriosclerosis
DNA Transposable Elements
DNA, Bacterial
Female
Humans
Male
Middle Aged
Polymerase Chain Reaction
Tuberculosis
Mycobacteriaceae
Mycobacterium
Mycobacterium tuberculosis complex
Abstract: The possible potential role of several infectious agents in atherosclerosis has been shown. Several infectious agents DNA in atheromatous plaques have been displayed by PCR. In patients with atheromas antibody levels against Hsp65 were higher. Vaccination of mice with recombinant Hsp65 and Hsp65-rich M. tuberculosis resulted in formation of atheromatous plaques. We attempted to detect M. tuberculosis DNA in atherosclerotic plaque samples by PCR. In endarterectomy tissue samples obtained from patients during coronary artery bypass graft surgery DNA was prepared by proteinase-K digestion, phenol/chloroform extraction and ethanol precipitation. After amplification with M.tuberculosis complex IS6110 region specific primers, the products were analyzed on electrophoresis. M. tuberculosis DNA was negative in all tissue samples. More data on etiological studies with mycobacteriaceae will be yield information on atherosclerosis pathogenesis.
URI: https://hdl.handle.net/11499/4907
ISSN: 1121-7138
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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