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https://hdl.handle.net/11499/4907
Title: | Mycobacterium tuberculosis complex DNA does not exist in atheromatous plaques | Authors: | Rota, Simin Tuncer, Serdar Kanat, O. |
Keywords: | Atherosclerosis M. tuberculosis PCR Phospholipid bacterial DNA BCG vaccine chloroform phenol primer DNA proteinase K adult article atherosclerotic plaque clinical article controlled study coronary artery atherosclerosis coronary artery bypass graft coronary artery disease electrophoresis endarterectomy extraction female human human tissue male Mycobacterium tuberculosis polymerase chain reaction precipitation tissue section Coronary Arteriosclerosis DNA Transposable Elements DNA, Bacterial Female Humans Male Middle Aged Polymerase Chain Reaction Tuberculosis Mycobacteriaceae Mycobacterium Mycobacterium tuberculosis complex |
Abstract: | The possible potential role of several infectious agents in atherosclerosis has been shown. Several infectious agents DNA in atheromatous plaques have been displayed by PCR. In patients with atheromas antibody levels against Hsp65 were higher. Vaccination of mice with recombinant Hsp65 and Hsp65-rich M. tuberculosis resulted in formation of atheromatous plaques. We attempted to detect M. tuberculosis DNA in atherosclerotic plaque samples by PCR. In endarterectomy tissue samples obtained from patients during coronary artery bypass graft surgery DNA was prepared by proteinase-K digestion, phenol/chloroform extraction and ethanol precipitation. After amplification with M.tuberculosis complex IS6110 region specific primers, the products were analyzed on electrophoresis. M. tuberculosis DNA was negative in all tissue samples. More data on etiological studies with mycobacteriaceae will be yield information on atherosclerosis pathogenesis. | URI: | https://hdl.handle.net/11499/4907 | ISSN: | 1121-7138 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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