Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/5016
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dc.contributor.authorDaumer, K.M.-
dc.contributor.authorTufan, Ahmet Çevik.-
dc.contributor.authorTuan, R.S.-
dc.date.accessioned2019-08-16T11:40:04Z
dc.date.available2019-08-16T11:40:04Z
dc.date.issued2004-
dc.identifier.issn0730-2312-
dc.identifier.urihttps://hdl.handle.net/11499/5016-
dc.identifier.urihttps://doi.org/10.1002/jcb.20190-
dc.description.abstractEndochondral skeletal development involves the condensation of mesenchymal cells, their differentiation into chondrocytes, followed by chondrocyte maturation, hypertrophy, and matrix mineralization, and replacement by osteoblasts. The Wnt family of secreted proteins have been shown to play important roles in vertebrate limb formation. To examine the role(s) of Wnt members and their transmembrane-spanning receptor(s), Frizzled (fz), we retrovirally misexpressed Wnt-5a, Wnt-7a, chicken frizzled-1 (Chfz-1), and frizzled-7 (Chfz-7) in long-term (21 day) high density, micromass cultures of stage 23/24 chick embryonic limb mesenchyme. This culture system recapitulates in vitro the entire differentiation (days 1-10), growth (days 5-12), and maturation and hypertrophy (from day 12 on) program of cartilage development. Wnt-7a misexpression severely inhibited chondrogenesis from day 7 onward. Wnt-5a misexpression resulted in a poor hypertrophic phenotype by day 14. Chfz-7 misexpression caused a slight delay of chondrocyte maturation based on histology, whereas Chfz-1 misexpression did not affect the chondrogenic phenotype. Misexpression of all Wnt members decreased collagen type X expression and alkaline phosphatase activity at day 21. Our findings implicate functional role(s) for Wnt signaling throughout embryonic cartilage development, and show the utility of the long-term in vitro limb mesenchyme culture system for such studies.en_US
dc.language.isoenen_US
dc.relation.ispartofJournal of Cellular Biochemistryen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject?-cateninen_US
dc.subjectChondrocyte hypertrophy and maturationen_US
dc.subjectChondrogenesisen_US
dc.subjectFrizzleden_US
dc.subjectN-cadherinen_US
dc.subjectWnt signalingen_US
dc.subjectfrizzled proteinen_US
dc.subjectprotein wnt 7aen_US
dc.subjectunclassified drugen_US
dc.subjectWnt1 proteinen_US
dc.subjectWnt5a proteinen_US
dc.subjectalkaline phosphataseen_US
dc.subjectbeta cateninen_US
dc.subjectcadherinen_US
dc.subjectcell surface receptoren_US
dc.subjectcollagen type 10en_US
dc.subjectcytoskeleton proteinen_US
dc.subjectsignal peptideen_US
dc.subjecttransactivator proteinen_US
dc.subjectWnt proteinen_US
dc.subjectanimal cellen_US
dc.subjectarticleen_US
dc.subjectcartilage cellen_US
dc.subjectcell differentiationen_US
dc.subjectcell maturationen_US
dc.subjectchondrogenesisen_US
dc.subjectembryoen_US
dc.subjectenzyme activityen_US
dc.subjectin vitro studyen_US
dc.subjectlimben_US
dc.subjectnonhumanen_US
dc.subjectpriority journalen_US
dc.subjectprotein expressionen_US
dc.subjectsignal transductionen_US
dc.subjectanimalen_US
dc.subjectcartilageen_US
dc.subjectchick embryoen_US
dc.subjectculture techniqueen_US
dc.subjectcytologyen_US
dc.subjectembryo developmenten_US
dc.subjectlimb buden_US
dc.subjectmetabolismen_US
dc.subjectphysiologyen_US
dc.subjectprenatal developmenten_US
dc.subjectVertebrataen_US
dc.subjectAlkaline Phosphataseen_US
dc.subjectAnimalsen_US
dc.subjectbeta Cateninen_US
dc.subjectCadherinsen_US
dc.subjectCartilageen_US
dc.subjectCell Culture Techniquesen_US
dc.subjectCell Differentiationen_US
dc.subjectChick Embryoen_US
dc.subjectCollagen Type Xen_US
dc.subjectCytoskeletal Proteinsen_US
dc.subjectEmbryonic Developmenten_US
dc.subjectIntercellular Signaling Peptides and Proteinsen_US
dc.subjectLimb Buden_US
dc.subjectReceptors, Cell Surfaceen_US
dc.subjectTrans-Activatorsen_US
dc.subjectWnt Proteinsen_US
dc.titleLong-term in vitro analysis of limb cartilage development: involvement of Wnt signalingen_US
dc.typeArticleen_US
dc.identifier.volume93en_US
dc.identifier.issue3en_US
dc.identifier.startpage526
dc.identifier.startpage526en_US
dc.identifier.endpage541en_US
dc.identifier.doi10.1002/jcb.20190-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.pmid15372624en_US
dc.identifier.scopus2-s2.0-16644390382en_US
dc.identifier.scopusqualityQ1-
dc.ownerPamukkale_University-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
item.fulltextNo Fulltext-
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
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