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https://hdl.handle.net/11499/50426
Title: | A combination of omega-3 and exercise reduces experimental Achilles tendinopathy induced with a type-1 collagenase in rats | Authors: | Gündoğdu, Gülşah Yılmaz Taşcı, Seymanur Gündoğdu, Köksal Terim Kapakın, Kübra Asena Demirkaya, Alper Kürşat Nalcı, Kemal Alp Gündoğdu, Mustafa Hacimuftuoglu, Ahmet El-Aty, A.M. Abd |
Keywords: | Achilles tendinopathy exercise omega-3 rat Pro-Inflammatory Cytokines Docosahexaenoic Acid Oxidative Stress Nitric-Oxide Fatty-Acids Apoptosis Tendons Modulation Tendinitis Responses |
Publisher: | Canadian Science Publishing | Abstract: | This study aimed to evaluate the effectiveness of omega-3 supplementation with exercise in a collagenase-induced Achilles tendinopathy (AT) rat model. Experimental groups (healthy control (HC), AT, exercise (Ex), omega-3 (W), and Ex+W) were randomly allocated. After a week of adaptation, oral omega-3 was initiated for 8 weeks (5 days/week). The exercise groups performed treadmill running for 30 min/day (5 days/week, 20 m/min, 8 weeks) following one week of adaptation (10 m/min, 15 min/day). Matrix metalloproteinase-13 (MMP-13), interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), and total antioxidant-oxidant status (Taş) levels were determined in serum samples. Tendon samples were obtained for biomechanical, histopathological, and immunohistochemical assessments. Ultimate tensile force, yield force, stiffness values, collagen type-I alpha 1 expression, and serum Taş significantly decreased (P < 0.05) in AT vs. HC. These values and expression significantly increased in the Ex+W group vs. AT. Serum MMP-13, IL-1 beta, and TNF-alpha levels decreased in all treatment groups vs. AT. The most significant decrease was found in the Ex+W group (P < 0.01). Histopathologically, the improvement in degeneration was statistically significant in the Ex+W group (P < 0.05). Immunohistochemically, MMP-13, IL-1 beta, TNF-alpha, and nitric oxide synthase2 expression was decreased in all treatment groups vs. AT. In conclusion, omega-3 and exercise might be recommended in AT patients. | Description: | Article; Early Access | URI: | https://doi.org/10.1139/apnm-2021-0801 https://hdl.handle.net/11499/50426 |
ISSN: | 1715-5312 1715-5320 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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