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https://hdl.handle.net/11499/5339| Title: | Detection of infectious bronchitis virus in tracheal organ cultures using the immunoperoxidase technique | Authors: | Şen, A. Sönmez, G. Caner, Vildan. Özyigit, M.O. |
Keywords: | Immunoperoxidase Infectious bronchitis virus Tracheal organ cultures formaldehyde paraffin peroxidase virus antigen animal disease animal tissue antigen detection article Avian infectious bronchitis virus chicken controlled study histopathology inoculation newborn nonhuman organ culture trachea tracheitis virus detection virus infection virus strain Animalia Aves Gallus gallus |
Abstract: | The applicability of the immunoperoxidase (IP) technique in unfixed-fresh tracheal organ cultures (TOCs) was investigated for rapid detection of infectious bronchitis virus (IBV). For this purpose, TOCs prepared from one-day-old SPF chicks were inoculated with IBV M41 strain in serial dilutions. IBV antigen detected by IP technique in TOCs 18 h postinoculation. IP staining was observed before siliostasis in all dilutions. The IP technique was also applied to detect IBV antigen in formalin-fixed, paraffin-embedded sections of TOCs inoculated with IBV M41 strain. Viral antigen was also detected in these sections. Histopathological examinations of IBV M41 infected tracheal rings showed loss of cilia, flattening of lamina epithelialis, and rounding and desquamation of epithelial cells. | URI: | https://hdl.handle.net/11499/5339 | ISSN: | 1300-0128 |
| Appears in Collections: | Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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