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https://hdl.handle.net/11499/5386
Title: | Detection of LOH of the RB1 gene in bladder cancers by PCR-RFLP | Authors: | Açıkbaş, İbrahim Keser, I. Kiliç, S. Bagcı, Hüseyin Karpuzoglu, G. Lüleci, G. |
Keywords: | Bladder neoplasm PCR-LOH assay PCR-RFLP Retinoblastoma gene article bladder carcinoma blood sampling cancer tissue controlled study cystitis gene amplification genetic analysis heterozygosity loss human human tissue intron major clinical study osteosarcoma polymerase chain reaction priority journal restriction fragment length polymorphism tumor suppressor gene Carcinoma, Transitional Cell Genes, Retinoblastoma Humans Loss of Heterozygosity Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Retinoblastoma Urinary Bladder Neoplasms |
Abstract: | Objectives: Retinoblastoma (RB1) gene involves in retinoblastoma, osteosarcoma, bladder, prostate, lung, breast carcinomas, and soft tissue sarcomas. Loss of heterozygosity (LOH) is the most common mutation of the gene. Methods: Xba I polymorphism in intron 17 of the gene was used to detect LOH in 20 bladder cancer patients. A cystitis and an osteosarcoma were used as control. LOH was investigated in three different kinds of samples (blood, paraffin-embedded tissue and fresh tissue) belonging to the same patients, and 20 blood samples, 20 paraffin-embedded tissue samples and 16 fresh tissue samples were obtained from 20 cancer patients. Results: None of the 20 blood samples showed LOH. Eleven out of 20 paraffin-embedded bladder tissues were amplified, 3 of them homozygous and all 8 informative paraffin-embedded tissues showed LOH. Five out of 16 fresh tumor tissues obtained were amplified, in 1 the fresh tissue was normal, 1 fresh tissue showed LOH and 3 were not digested by Xba I. Conclusion: The results of the study have suggested that detection of LOH of the RB1 gene by PCR-RFLP can be a good adjunctive test for evaluation of the bladder cancer. Copyright © 2002 S. Karger AG, Basel. | URI: | https://hdl.handle.net/11499/5386 https://doi.org/10.1159/000048448 |
ISSN: | 0042-1138 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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