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https://hdl.handle.net/11499/5438
Title: | Cloning, sequencing, and characterization of CYP1A1 cDNA from leaping mullet (Liza Saliens) liver and implications for the potential functions of its conserved amino acids | Authors: | Şen, Alaattin Hu, C.-H. Urbach, E. Wang-Buhler, J.-L. Yang, Y.-H. Arinc, E. Buhler, D.R. |
Keywords: | Cloning CYP1A1 Leaping mullet Liza Saliens Phylogenetic analysis adenine amino acid complementary DNA cytochrome P450 1A1 cytosine guanine thymine 3' untranslated region aquatic fauna article binding site controlled study DNA flanking region DNA library DNA probe enzyme analysis enzyme substrate exon fish gene gene insertion gene sequence genetic code genetic procedures genetic screening human intron liver mammal methodology molecular cloning nonhuman nucleotide sequence open reading frame phylogeny prediction protein motif protein secondary structure reporter gene seashore Southern blotting species species difference Turkey (republic) vertebrate Amino Acid Sequence Amino Acids Animals Base Sequence Blotting, Northern Blotting, Southern Cloning, Molecular Cytochrome P-450 CYP1A1 DNA, Complementary Humans Liver Molecular Sequence Data Phylogeny Protein Structure, Secondary Sequence Homology, Amino Acid Smegmamorpha Liza saliens Mammalia Oncorhynchus mykiss Salmonidae Vertebrata |
Abstract: | A 2,037 bp CYP1A1 cDNA (GenBank AF072899) was cloned through screening of a ?ZipLox cDNA library constructed from the liver of a leaping mullet (Liza saliens) fish captured from Izmir Bay on the Aegean coast of Turkey using rainbow trout CYP1A1 cDNA as a probe. This clone has a 130 bp 5'-flanking region, a 1,563 bp open reading frame (ORF) encoding a 521-amino acid protein (58,972 Da), and a 344 bp 3'-untranslated region without a poly (A) tail. Alignment of the deduced amino acids of CYP1A1 cDNAs showed 58% and 69-96% identities with human and 12 other fish species, respectively. Southern blot analysis suggested that this CYP1A1 cDNA was from a single-copy gene. Based on the comparison with CYP1A1 genes reported for fish and mammals, the leaping mullet CYP1A1 gene is probably split into 7 exons. The intron insertion sites were predicted. Alignment of the CYP1A1 cDNA encoded amino acids from 13 fish and 7 mammalian species disclosed differences in highly conserved amino acids between aquatic and land vertebrates. The possible associated secondary structure; conserved motifs and substrate-binding sites were discussed. The phylogenetic relationships of CYP1A1s among 13 fish species were analyzed by a distance method. © 2001 John Wiley & Sons, Inc. | URI: | https://hdl.handle.net/11499/5438 https://doi.org/10.1002/jbt.10005 |
ISSN: | 1095-6670 |
Appears in Collections: | Fen-Edebiyat Fakültesi Koleksiyonu PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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