Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/5438
Title: Cloning, sequencing, and characterization of CYP1A1 cDNA from leaping mullet (Liza Saliens) liver and implications for the potential functions of its conserved amino acids
Authors: Şen, Alaattin
Hu, C.-H.
Urbach, E.
Wang-Buhler, J.-L.
Yang, Y.-H.
Arinc, E.
Buhler, D.R.
Keywords: Cloning
CYP1A1
Leaping mullet
Liza Saliens
Phylogenetic analysis
adenine
amino acid
complementary DNA
cytochrome P450 1A1
cytosine
guanine
thymine
3' untranslated region
aquatic fauna
article
binding site
controlled study
DNA flanking region
DNA library
DNA probe
enzyme analysis
enzyme substrate
exon
fish
gene
gene insertion
gene sequence
genetic code
genetic procedures
genetic screening
human
intron
liver
mammal
methodology
molecular cloning
nonhuman
nucleotide sequence
open reading frame
phylogeny
prediction
protein motif
protein secondary structure
reporter gene
seashore
Southern blotting
species
species difference
Turkey (republic)
vertebrate
Amino Acid Sequence
Amino Acids
Animals
Base Sequence
Blotting, Northern
Blotting, Southern
Cloning, Molecular
Cytochrome P-450 CYP1A1
DNA, Complementary
Humans
Liver
Molecular Sequence Data
Phylogeny
Protein Structure, Secondary
Sequence Homology, Amino Acid
Smegmamorpha
Liza saliens
Mammalia
Oncorhynchus mykiss
Salmonidae
Vertebrata
Abstract: A 2,037 bp CYP1A1 cDNA (GenBank AF072899) was cloned through screening of a ?ZipLox cDNA library constructed from the liver of a leaping mullet (Liza saliens) fish captured from Izmir Bay on the Aegean coast of Turkey using rainbow trout CYP1A1 cDNA as a probe. This clone has a 130 bp 5'-flanking region, a 1,563 bp open reading frame (ORF) encoding a 521-amino acid protein (58,972 Da), and a 344 bp 3'-untranslated region without a poly (A) tail. Alignment of the deduced amino acids of CYP1A1 cDNAs showed 58% and 69-96% identities with human and 12 other fish species, respectively. Southern blot analysis suggested that this CYP1A1 cDNA was from a single-copy gene. Based on the comparison with CYP1A1 genes reported for fish and mammals, the leaping mullet CYP1A1 gene is probably split into 7 exons. The intron insertion sites were predicted. Alignment of the CYP1A1 cDNA encoded amino acids from 13 fish and 7 mammalian species disclosed differences in highly conserved amino acids between aquatic and land vertebrates. The possible associated secondary structure; conserved motifs and substrate-binding sites were discussed. The phylogenetic relationships of CYP1A1s among 13 fish species were analyzed by a distance method. © 2001 John Wiley & Sons, Inc.
URI: https://hdl.handle.net/11499/5438
https://doi.org/10.1002/jbt.10005
ISSN: 1095-6670
Appears in Collections:Fen-Edebiyat Fakültesi Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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