The renal effects of ten hour sevoflurane anaesthesia in rats: The role of soda lime

Abstract

The aim of this study was to evaluate the effects of sevoflurane anaesthesia on renal functions by conducting with or without soda lime comparatively. Eighteen male Wistar rats were divided into three equal groups randomly. Sevoflurane in 1.5 % concentration were administered directly to first group in 4 L/min fresh gas flow (50 % O2 + 50 % N2O), while the second group were applied the same gas mixture through soda lime, that is exposed to 150 mL/min carbon dioxide. Third group were control group, breathing room air. Glucose in blood specimens at the begining and end of application and glucose, gamma glutamyl transferase and protein levels were measured in urine samples collected during application period. Renal tissue specimens were evaluated histopathologically. Groups were similar with respect to blood glucose levels. Although urine glucose levels were higher in both of two study groups compared to control (p<0.00001), there was no significant difference between them. Mean urinary GGT level was reasonably high in sevoflurane group, that was not used soda lime, with respect to other two groups (p<0.0004). Mean urine protein levels were similar in all groups. Inflammatory cell collections were observed in the renal tissue specimens of study groups. It was concluded that long term sevoflurane anaesthesia can have toxic effects on renal tissue of rats; and in vivo metabolites may have greater role than in vitro ones, that result from reaction with soda lime.