Electrocatalytic Determination of Uric Acid with the Poly(Tartrazine)-Modified Pencil Graphite Electrode in Human Serum and Artificial Urine

Abstract

A novel electrocatalytic sensing strategy was built for uric acid (UA) determination with an exceptionally developed poly(tartrazine)-modified activated pencil graphite electrode (pTRT/aPGE) in human serum and artificial urine. The oxidation signal of UA at 275 mV in pH 7.5 phosphate buffer solution served as the analytical response. Cyclic voltammetry, electrochemical impedance spectroscopy, scanning electron microscopy, energydispersive X-ray spectroscopy, and X-ray photoelectron spectroscopy were used to characterize the sensing platform, which was able to detect 0.10 mu M of UA in the ranges of 0.34-60 and 70-140 mu M. The samples of human serum and artificial urine were analyzed by both the pTRT/aPGE and the uricase-modified screen-printed electrode. The results were statistically evaluated and compared with each other within the confidence level of 95%, and no significant difference between the results was found.